波利多卡诺尔通过准FSR的定数感应系统来抑制Enterococcus faecalis的毒性因素
Dina Ashraf1, Mona I Shaaban2, Ramadan Hassan1
1Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.
BMC microbiology
|October 16, 2024
概括
波利多卡诺尔有效地向Enterococcus faecalis中的fsr定数感应系统,减少毒性因素和生物膜形成. 这为抗生素耐药性E. faecalis感染提供了一个有前途的策略.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 药理学 药理学是指药理学的学科.
背景情况:
- Enterococcus faecalis的抗菌耐药性是一个重大的全球卫生挑战.
- 对于E. faecalis的致病性和毒性因子调节来说,fsr的定数感应 (QS) 系统至关重要.
- 准fsr QS系统是对抗抗生素耐药性E. faecalis感染的潜在策略.
研究的目的:
- 调查化合物对E. faecalis. fsr QS系统的活性.
- 评估潜在的QS抑制剂对E. faecalis毒性因子的影响.
- 评估波利多卡诺尔作为对抗E. faecalis感染的治疗剂.
主要方法:
- 分析了60个临床E. faecalis分离物,以检测毒性因素.
- 66种化合物被选为对FSRQS的活性.
- 最小抑制度 (MICs) 通过微乳液稀释来确定.
- 亚抑制度对QS和毒性基因的影响使用凝酶试验和定量实时PCR进行了评估.
主要成果:
- 聚多醇显著降低了凝酶和蛋白酶的产生 (54-85%).
- 聚多卡诺尔抑制了生物膜的形成,并破坏了成熟的生物膜.
- 聚多卡诺尔降低了细胞表面的疏水性,并降低了关键的QS和病毒性基因 (fsrB,fsrC,gelE,sprE,asa1,epbA) 的下调,而不会影响细菌的活力.
- 聚多卡诺尔在低于致命的度下表现出显著的抗病毒活性.
结论:
- 波利多卡诺尔是治疗E. faecalis感染的有前途的治疗候选药物.
- 波利多卡诺尔有效地准FSR QS系统,并表现出抗生物膜特性.
- 这项研究凸显了聚多卡诺尔在克服E. faecalis.抗生素耐药性的潜力.
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