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在表皮细胞中,CHUP1限制了叶绿体的运动和效应因子触发的免疫力

Alexander O Nedo^1,2,3, Huining Liang⁴, Jaya Sriram^1,2

¹Department of Plant and Soil Sciences, University of Delaware, Newark, DE, 19716, USA.

The New phytologist

|October 17, 2024

概括

叶绿体异常定位1 (CHUP1) 固叶绿体. 在表皮细胞中抑制CHUP1增强了防御反应和免疫力,揭示了叶绿体的定.

科学领域:

植物细胞生物学植物细胞生物学
植物免疫力植物免疫力
分子植物科学分子植物科学

背景情况:

叶绿体异常定位1 (CHUP1) 调节叶绿体在美索菲尔细胞中的运动.
在表皮细胞中,CHUP1沉默会诱导叶绿细胞流体,并增强效应因子触发免疫力 (ETI).
将CHUP1的功能与表皮细胞中的ETI联系在一起的确切机制尚不清楚.

研究的目的:

研究CHUP1的双重功能 (定和运动调节) 在表皮细胞内的ETI中的作用.
在CHUP1调制的反应中量化叶绿体定和运动动态.
为了阐明叶绿体脱和过氧化 (H2O2) 对ETI的贡献.

主要方法:

在Nicotiana benthamiana表皮细胞中对叶绿体的定和运动进行定量分析.
对NbCHUP1的基因沉默,以评估其对叶绿体行为和防御反应的影响.
测量表皮质防御 (ECD) 反应,包括形形成,周核聚类,表皮质反应 (ECR) 和活性氧物种 (ROS) 生产 (H2O2).

主要成果:

沉默NbCHUP1导致表皮层质体脱和基底运动增加,但没有完全取消蓝光诱导的运动.
沉默NbCHUP1触发了多个ECD反应:流体形成,周核质细胞聚类,ECR和H2O2产生.
发现,质体的定限制了这些多面的ECD反应.
积累的叶绿素H2O2对于增加的叶绿素和增强的ETI是必不可少的,但对于增加的基底叶绿素运动不是必不可少的.

结论:

叶绿体的定作用是对复杂的表皮叶绿体防御机制的限制.
质体脱和H2O2积累在ETI的增加中发挥着不同的但至关重要的作用.
CHUP1在叶绿体固中的作用对于调节表皮细胞中的植物防御反应至关重要.

关键词:

хлоропласт 不寻常的定位 1 (CHUP1)叶绿体运动叶绿体运动作用因子触发免疫力 (ETI)皮肤表层质体防御 (ECD)皮肤上的质体反应 (ECR)摄影蛋白2 (phot2) 的使用有活性氧物种的反应性氧物种.它们是Stromules,Stromules.

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