便携式微型质谱仪用于通过整合固相微提取和纳米电子喷雾电离的复杂样品中增强现场检测分析物
Xuan Liu1, Baixue Wang1, Haiyan Luo2
1College of Environment and Climate, Institute of Mass Spectrometry and Atmospheric Environment, Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution, and Guangdong Provincial Key Laboratory of Speed Capability, Jinan University, Guangzhou 510632, China.
Analytical chemistry
|October 17, 2024
概括
一个新的便携式质谱 (MS) 套件结合了固相微提取 (SPME) 与纳米电子喷雾电离 (nESI) 进行现场化学分析. 这种创新方法可以在各种真实世界样本中灵敏,快速检测化合物.
科学领域:
- 分析化学 分析化学
- 环境科学 环境科学
- 生物技术是生物技术.
背景情况:
- 现场质谱 (MS) 对于现场样本分析至关重要,但在小型化,便携性和灵敏性方面面临挑战.
- 现有的方法通常需要复杂的样品准备和基于实验室的仪器仪表,限制了快速的现场评估.
研究的目的:
- 开发一种便携式和敏感的质谱 (MS) 方法,用于现场分析复杂样品.
- 将生物相容的固相微提取 (SPME) 与纳米电子喷雾电离 (nESI) 结合起来,以与微型MS (mMS) 进行合.
主要方法:
- 开发一个集成SPME纤维用于提取取样的套件,与nESI发射器用于直接合到微型MS (mMS).
- 用于在现场从液体样本和生物体中提取分析物的SPME.
- 采用nESI用于通过集成套件直接MS分析提取的分析物.
主要成果:
- 在测试化合物的每毫升 (pg/mL) 皮克克的水平上达到检测极限.
- 在复杂矩阵中进行直接测量时,证明了可接受的相对标准偏差 (RSD) 值 (5.57.6%).
- 确认了可接受的线性反应 (0.150 ng/mL) 和矩阵效应 (76.082.6%).
- 在食品,人体液体,环境水和生物体中成功增强了目标化合物的检测.
结论:
- 开发的SPME-nESI-mMS系统是对各种复杂样本的现场分析的一个有前途的工具.
- 这种方法为食品安全,环境监测,法医调查和生物分析领域的应用提供了巨大的潜力.
- 将SPME和nESI与mMS集成,为现场化学分析提供了灵敏,便携和高效的解决方案.
相关概念视频
High-Performance Liquid Chromatography: Types of Detectors
496
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
496
Electrospray Ionization (ESI) Mass Spectrometry
743
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
743
Mass Spectrometry: Complex Analysis
732
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
732
Mass Spectrometers
5.2K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.2K
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
657
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
657
Gas Chromatography: Types of Detectors-II
338
In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
338


