抗真菌化合物的生产优化的发酵由菌 velezensis LZN01和转录组分析
Jiale Hu1,2,3, Zhigang Wang1,2,3, Weihui Xu1,2,3
1College of Life Science and Agroforestry, Qiqihar University, Qiqihar, China.
Microbial biotechnology
|October 17, 2024
概括
优化Bacillus velezensis LZN01的发酵条件显著提高了其对Fusarium oxysporum f. sp.的抗真菌活性. (Fon),增强西瓜疾病管理.
科学领域:
- 农业科学 农业科学
- 微生物学 微生物学
- 植物病理学 植物病理学
背景情况:
- 虫,是由虫 (Fusarium oxysporum f. sp.) 引起的 (Fon),严重影响全球西瓜产量和质量.
- 反对性的细菌提供了一种实际的生物控制策略,用于管理西瓜中的Fusarium枯.
研究的目的:
- 通过发酵优化,最大限度地提高Bacillus velezensis LZN01对Fon的抗真菌活性.
- 通过转录组测序分析增强抗真菌活性背后的调节机制.
主要方法:
- 使用单因子和响应表面实验,优化了Bacillus velezensis LZN01的培养和发酵条件.
- 通过转录组测序分析基因表达变化.
- 使用代谢学和UPLC/Q-Exactive Orbitrap MS.使用量化抗菌化合物生产.
主要成果:
- 最佳条件 (35 g/L D-果糖,5 g/L NH4Cl,pH 7,30°C,72h) 将真菌抑制率提高到71.1%.
- 转录组分析揭示了参与代谢和抗微生物化合物合成的基因的显著上调和下调.
- 关键抗微生物化合物 (表面素,素,石基胺酸,素) 的生产产量在优化后增加.
结论:
- 发酵的优化显著提高了Bacillus velezensis LZN01对Fusarium病原体的抗真菌功效.
- 增强的活性归因于与抗微生物化合物生物合成相关的基因表达的调节.
- 这种优化的菌株在西瓜种植中具有可持续Fusarium枯管理的潜力.
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