酵母基因ECM9在压力条件下调节细胞壁维护和细胞分裂
Prevena Ramakrishnan1, Jill Keeney1
1Biology, Juniata College, Huntingdon, Pennsylvania, United States.
microPublication biology
|October 17, 2024
概括
这项研究表明,Saccharomyces cerevisiae中的ECM9基因对于维持细胞壁完整性和在压力下调节细胞分裂至关重要. 删除ECM9可能会影响酵母.
科学领域:
- 分子和细胞生物学分子和细胞生物学
- 酵母遗传学 酵母遗传学
- 简介:真核细胞的功能
背景情况:
- 细菌菌 (Saccharomyces cerevisiae) 是真核细胞生物学的一个模型生物体.
- 尽管进行了广泛的研究,但近10%的酵母基因,包括YKR004C (ECM9),仍然没有表征.
- 以前预测ECM9在细胞分裂和细胞壁组成中发挥作用.
研究的目的:
- 为了研究Saccharomyces cerevisiae中未表征的ECM9基因的功能.
- 确定ECM9在细胞壁维护和应激条件的反应中的作用.
- 阐明ECM9在压力下的细胞分裂调节中的参与.
主要方法:
- 一种ECM9缺失菌株与野生型Saccharomyces cerevisiae的比较.
- 在各种压力条件下评估细胞壁完整性.
- 对应环境压力的细胞分裂调节的分析.
主要成果:
- 与野生类型相比,ECM9删除菌株对细胞壁应激的敏感性增加.
- ECM9在维持细胞壁完整性方面发挥着重要作用.
- ECM9涉及控制应激过程中细胞分裂准备的调节通路.
结论:
- 在Saccharomyces cerevisiae中,ECM9基因对于坚固的细胞壁维护至关重要.
- ECM9是控制细胞分裂的应激反应途径的关键组成部分.
- 了解ECM9功能,可以了解真核细胞细胞分裂和细胞壁动态.
相关概念视频
Yeast Signaling
14.4K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
14.4K
Role of Microtubules in Cell Wall Deposition
2.3K
Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of...
2.3K
Molecular Factors Affecting Cell Division
3.0K
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
3.0K
Cells Coordinate Growth and Proliferation
4.5K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
4.5K
Maintenance of the ES Cell State
2.2K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.2K
Bacterial Cell Wall
3
The bacterial cell wall is an essential structural component that encases the plasma membrane, preserving cellular integrity, determining shape, and protecting against osmotic stress. This rigid yet flexible structure primarily comprises peptidoglycan, a polymer that forms a mesh-like matrix conferring mechanical strength and flexibility.Peptidoglycan Composition and StructurePeptidoglycan, the core of the bacterial cell wall, comprises alternating units of N-acetylglucosamine (NAG) and...
3


