肠道细菌通过减少性OSrABC途径代谢天然和合成类固醇激素
Christian Jacoby1,2, Kaylie Scorza1,2, Lia Ecker1,2
1Duchossois Family Institute, University of Chicago, Chicago, IL, USA.
bioRxiv : the preprint server for biology
|October 17, 2024
概括
一种新的肠道细菌,Clostridium steroidoreducens,可以代谢类固醇激素. 它的酶,OsrABC,降低皮质醇和合成皮质类固醇,可能影响诸如克罗恩氏症等炎症性疾病.
科学领域:
- 微生物学 微生物学
- 内分泌学 在内分泌学.
- 肠道微生物组研究研究
背景情况:
- 人们对肠道微生物在类固醇激素代谢中的作用知之甚少.
- 激素的微生物转化影响宿主生理和疾病.
研究的目的:
- 为了识别和描述参与类固醇激素代谢的肠道细菌.
- 阐明负责降低类固醇激素的酶途径.
- 研究微生物类固醇代谢与炎症性肠病之间的联系.
主要方法:
- 一种新的肠道细菌的分离和表征, *Clostridium steroidoreducens* HCS.1.
- 转录组分析以确定相关的基因和途径.
- 关键酶 (OsrA,OsrB,OsrC) 的异质表达和生物化学表征.
- 微生物基因组和元基因组数据的生物信息分析.
主要成果:
- *Clostridium steroidoreducens* HCS.1 将主要的类固醇激素 (皮质醇,孕,) 降低为四类固醇产物.
- OsrABC途径调解了降低性类固醇激素代谢,而OsrA针对的是合成皮质类固醇.
- *osrA*和*osrB*的同源体在各种肠道细菌中发现,并且在克罗恩病元基因组中得到丰富.
结论:
- 确定了OsrABC通路作为肠道中降解性类固醇激素代谢的基础.
- 证明微生物酶可以耗尽抗炎性皮质类固醇.
- 建立了肠道微生物类固醇代谢与克罗恩病等炎症性疾病之间的潜在联系.
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