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相关概念视频

RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K
DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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相关实验视频

Updated: Jun 10, 2025

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
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2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

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通过近距离条形码测序同时映射多个RNA修改.

Erdem Sendinc1,2,3, Hua Yu4, Yu Hsien Hwang Fu4

  • 1Stem Cell Program, Division of Hematology/Oncology, Boston Children's Hospital, Boston, MA 02115, USA.

bioRxiv : the preprint server for biology
|October 17, 2024
PubMed
概括
此摘要是机器生成的。

通过EpiPlex RNA修饰分析,可以同时绘制多个RNA标记,从而推进表体转录组研究. 这种新方法揭示了METTL3和EIF4A3如何影响N6-甲基氨酸 (m6A) 水平和分布.

关键词:
欧洲投资基金4A3A3在METTL3中,METTL3是METTL3的第一个类型.基因组RNA的修饰 基因组RNA的修饰副本转录组 (epitranscriptome) 是一个副本转录组 (epitranscriptome).没有 inosine inosine 的问题.m6A 一个很好的.

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Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

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相关实验视频

Last Updated: Jun 10, 2025

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
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2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

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Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物化学 生化学

背景情况:

  • 表体转录组包括多种RNA化学修饰,mRNA上的N6-甲基氨酸 (m6A) 对于转录后基因调节至关重要.
  • 现有的技术允许单个RNA修饰的转录组范围的映射,但缺乏同时进行多修饰分析的方法.
  • 在单个样本中同时检测多个RNA修饰是表表表转录组研究中的一个重大未满足的需求.

研究的目的:

  • 开发一种新,方便的方法,同时绘制多个RNA修饰的地图.
  • 为了使RNA修饰丰度在特定位置的相对量化.
  • 研究METTL3和EIF4A3对表观转录组的影响.

主要方法:

  • EpiPlex RNA修饰分析,一个基于珠子的近距离条形码测定与测序读出.
  • 使用分子识别来检测低RNA输入的多目标RNA修饰.
  • 通过测量与spike-in控制器相比的信号强度来实现相对量化.

主要成果:

  • EpiPlex成功地同时绘制了多个RNA修饰的地图.
  • 药物抑制METTL3降低了m6A水平,而EIF4A3抑制/敲击增加了m6A水平.
  • EIF4A3操纵证实了它在塑造美景中的作用,而不会影响伊诺辛水平.

结论:

  • EpiPlex提供了一个可靠和方便的平台,用于同时绘制多个RNA修饰的地图.
  • 该方法有助于更深入地了解表皮转录组的动态和调节.
  • 这些发现阐明了METTL3和EIF4A3在调节m6A修改模式中的作用.