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从原发性硬化胆管炎患者分离的培养细菌诱导胆汁炎症和细胞死亡

Chelsea E Powell, Megan D McCurry, Silvia Fernanda Quevedo

bioRxiv : the preprint server for biology

|October 17, 2024

概括

在原发性硬化性胆道炎 (PSC) 患者的胆汁中发现的细菌诱导细胞变化,可能导致肝脏疾病. 特定的细菌可能会增加肠道的透性,并导致胆道树的炎症.

科学领域:

肝病学肝病学是一种肝病学.
微生物学微生物学
胃肠病学胃肠病学

背景情况:

原发性硬化胆炎 (PSC) 是一种慢性肝病,原因不明.
已经观察到肠道微生物组和PSC之间的关联.
目前PSC的治疗选择有限.

研究的目的:

调查PSC患者胆汁中的细菌是否可以诱导与疾病相关的细胞变化.
为了识别特定的细菌菌株及其对肝脏和胆道细胞的影响.

主要方法:

从PSC患者和对照组中采集了胆汁样本.
胆汁细菌被培养并使用16SrRNA测序识别.
来自PSC相关细菌菌株的超级活体在人类结肠,肝脏和胆道细胞上进行了测试.

主要成果:

从大多数PSC患者中分离出细菌,但没有对照.
在PSC胆汁微生物群中,多样性减少,菌株占主导地位.
与PSC相关的细菌诱导了细胞毒性,上皮透性和细胞中的炎症性细胞因子释放.

结论:

从PSC胆汁培养的细菌可以诱导细胞变化,从而导致疾病的发病.
肠球菌可能会增加肠道的透性,促进细菌进入胆道树.
埃舍里希亚菌,Fusobacterium和Klebsiella可能会导致胆道和肝细胞的炎症和损伤.

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