聚亚丁酶PAPI是需要在致病性细菌中维护毒性等离子体的
Katherine Schubert1, Micah Braly2, Jessica Zhang3
1Department of Molecular, Cell, and Developmental Biology, UC Santa Cruz, Santa Cruz, CA 95064, United States.
bioRxiv : the preprint server for biology
|October 17, 2024
概括
由细菌基因pcnB编码的多基酶PAP I对于维持病原体中的毒性和抗生素耐药性等离子体至关重要. 这种酶稳定了等离子体,确保了关键基因的强大表达,增强了细菌的毒性和抗生素耐药性.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 病原细菌利用等离子体编码的毒性因子来感染.
- 塑体复制和复制数的调节对于毒性基因表达至关重要.
- 耶尔西尼亚的III型分泌系统 (T3SS) 对于毒性至关重要,并被编码在pYV等离子体上.
研究的目的:
- 研究染色体基因pcnB的作用,编码多基酶PAP I,在调节等离子体复制数 (PCN) 和Yersinia中的毒性.
- 确定pcnB/PAP I是否影响细菌病原体中的毒性和抗微生物耐药性 (AMR) 质体的稳定性和表达.
主要方法:
- 研究了pcnB/PAP I在Yersinia和Shigella菌株中的功能.
- 评估了pcnB/PAP I对pYV等离子体拷贝数和T3SS活性的影响.
- 使用小鼠感染模型评估了pcnB/PAP I在细菌毒性中的作用.
- 研究了pcnB/PAP I对PCN的影响以及sRNA调节的抗微生物耐药性等离子体的表达.
主要成果:
- 编码PAP I的染色体基因pcnB对于调节pYV等离子体拷贝数,维持pYV稳定性,强大的T3SS活性和Yersinia毒性至关重要.
- 在Shigella flexneri中,pcnB/PAP I也是T3SS表达的必要条件.
- pcnB/PAP I维持了抗微生物耐药性塑模型的正常PCN,增加了抗生素耐药性的十倍.
- pcnB/PAP I在细菌中广泛存在,但研究不足.
结论:
- pcnB/PAP I在细菌病原体中病毒性和抗菌素耐药性等离子体的传播和稳定中发挥着至关重要的作用.
- 这种酶对于维持等离子体编码特征所需的基因剂量至关重要,影响细菌毒性和抗生素耐药性.
- 这些发现凸显了pcnB/PAP I在打击抗生素耐药性的潜在重要性,并强调了它在增强病原细菌等离子体稳定性和毒性方面的作用.
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