由Toxoplasma gondii引起的入侵孔
Y Kegawa1, F Male2, I Jiménez-Munguía1
1Section on Integrative Biophysics; Division of Basic and Translational Biophysics, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), National Institutes of Health (NIH).
bioRxiv : the preprint server for biology
|October 17, 2024
概括
毒素菌使用一种
科学领域:
- 寄生虫学的寄生虫学
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 强制性细胞内寄生虫如Toxoplasma gondii侵入宿主细胞以求生存.
- 在接触后,Toxoplasma gondii将必要的入侵蛋白注入宿主细胞.
研究的目的:
- 为了研究东道细胞侵袭的生物物理机制,由Toxoplasma gondii.
- 描述寄生虫入侵期间宿主细胞膜导电性的短暂变化.
主要方法:
- 利用暴露于Toxoplasma gondii的宿主细胞的高分辨率电生理学记录 (小于200毫秒).
- 应用时间序列分析对一大批寄生虫诱导的膜导电过渡的数据集.
- 研究了RON2蛋白在侵袭和导电性变化的作用,使用枯竭的寄生虫.
主要成果:
- 检测到宿主膜导电率在Toxoplasma gondii入侵之前短暂增加.
- 寄生虫缺少RON2诱导导电过渡物,但未能侵入,表明RON2不是直接结构性的.
- 揭示了瞬态内部的多个量子导电性步骤,表明孔隙形成和调制.
- 确定了类似于EXP2蛋白转位的导电性步骤大小,提出了"入侵孔"一词.
结论:
- 毒素菌对宿主细胞的入侵涉及一种短暂的量子导电路,称为"入侵孔".
- 蛋白质转位体EXP2是这种入侵孔的潜在组成部分.
- RON2在侵入过程中起作用,但在导电通路的直接形成或封闭中没有作用.
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