H2AK119ub的动态控制了毛囊干细胞静止的情况
bioRxiv : the preprint server for biology
|October 17, 2024
概括
压制性组织蛋白标记保持干细胞静止. 毛囊干细胞 (HFSCs) 中H2AK119ub的丢失导致过早激活和耗尽,揭示了组织再生的表观遗传控制.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 干细胞生物学 干细胞生物学
- 皮肤病学 皮肤病学
背景情况:
- 干细胞激活涉及拆除静止程序并建立细胞循环促进.
- 表观遗传调节在通过干细胞状态的成年组织再生中的作用尚未完全理解.
研究的目的:
- 调查压制性组织蛋白修饰H2AK119ub在调节毛囊干细胞 (HFSC) 静止和激活中的作用.
- 阐明表观遗传过程在组织再生过程中控制干细胞功能的机制.
主要方法:
- 在成年小鼠皮肤中静止和活跃的HFSC中分析动态H2AK119ub水平.
- 在HFSC中对H2AK119ub进行基因切除,以评估其对静止和干细胞池的影响.
- 转录和染色质研究以确定基因和调节途径.
- 调查利基信号,特别是FGF信号,以维持H2AK119ub水平.
主要成果:
- H2AK119ub在静止和活跃的HFSC之间进行动态调节.
- 切除H2AK119ub会导致HFSC静止受损,过早激活,并最终耗尽干细胞池.
- H2AK119ub直接抑制了一种转录程序,该程序促进HFSC中的增殖.
- 来自利基角质细胞的抑制FGF信号维持H2AK119ub在静止的HFSC中.
结论:
- 压制性组织蛋白标记H2AK119ub对于维持HFSC静止至关重要.
- 通过利基信号的动态调节H2AK119ub可以防止干细胞过早激活,从而保持干细胞的功能和寿命.
- 通过H2AK119ub进行表观遗传控制对于成人组织再生和干细胞维护至关重要.
相关概念视频
01:06Multipotency and Niche of Bulge Stem Cell
3.5K
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
3.5K
01:19Multipotency of Hematopoietic Stem Cells
3.0K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.0K
01:14Maintenance of the ES Cell State
2.2K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.2K
01:12Renewal of Skin Epidermal Stem Cells
2.5K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.5K
01:26Stem Cell Niche
5.0K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
5.0K
01:32Chromatin Modification in iPS Cells
1.6K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.6K