特定物种的卫星DNA组成决定了PRC1-介导的中心异质染色蛋白
Piero Lamelza1, Malena Parrado1, Michael A Lampson1
1Department of Biology, University of Pennsylvania, Philadelphia, PA, 19104.
bioRxiv : the preprint server for biology
|October 17, 2024
概括
卫星DNA序列的变化影响了早期胚胎异染色素的形成. 不同的序列影响了多抑制复合体1 (PRC1) 的结合,影响了染色体分离和线粒状相互作用.
科学领域:
- 表观遗传学和发育生物学
- 基因组学和分子进化
背景情况:
- 周心体对染色体分离至关重要,但含有快速演变的卫星DNA.
- 像H3K9me3这样的表观遗传标记保持了异染色素,但在小鼠胚胎中缺少父性染色素.
- 卫星DNA序列变异对早期异色染色体形成的影响尚不清楚.
研究的目的:
- 为了研究卫星DNA变异对周边中心的异色素蛋白形成的功能后果.
- 确定卫星DNA序列变异如何影响染色体乘客综合体 (CPC) 的招募.
- 分析卫星DNA变异对与线粒状互动的影响.
主要方法:
- 使用其他老鼠物种的精子与*M. musculus*蛋的物种间受精.
- 研究了多镇压复合体1 (PRC1) 的AT子与不同的卫星DNA序列的结合.
- 评估了H2AK119ub1在线粒染色体上的异性染色体的形成.
- 分析了CPC招募途径和微管体对动态细胞的力量.
主要成果:
- PRC1的AT直接识别A/T丰富的卫星DNA,形成H2AK119ub1异染色蛋白.
- 不同的卫星序列表现出不同的结合PRC1的能力,导致差异H2AK119ub1异质染色素的形成.
- 强大的H2AK119ub1异色染色体形成抑制了CPC的招募,增加了微管体对kinetochores的力量.
结论:
- 卫星DNA的组成直接影响着周心层的功能.
- 早期胚胎生成对卫星DNA进化敏感,影响染色体分离.
- 通过PRC1介导的H2AK119ub1异色染色体的形成是将卫星DNA变异与周心细胞功能联系起来的关键机制.
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