聚细菌细胞内宏分子的形状 单细胞的Epikine 档案 在上呼吸道 粘液
Quinn T Easter1, Zabdiel Alvarado-Martinez1, Meik Kunz2
1Lab of Oral & Craniofacial Innovation (LOCI), ADA Science & Research Institute, Gaithersburg, MD, USA.
bioRxiv : the preprint server for biology
|October 17, 2024
概括
细菌分子存在于口腔细胞中,影响免疫反应和药物疗效. 了解这些宿主-微生物相互作用是健康和疾病管理的关键.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 细胞生物学 细胞生物学
背景情况:
- 上呼吸道上皮质作为对微生物的屏障.
- 鼻腔和口腔粘膜部位容易发生失生症和炎症.
- 这些组织中的宿主微生物相互作用对健康至关重要.
研究的目的:
- 为了研究人类口腔粘膜上皮细胞内的宿主微生物相互作用.
- 识别细菌特征及其对表皮细胞反应的影响.
- 探索持久性细菌大分子在细胞功能和药物反应中的作用.
主要方法:
- 从人类口腔粘膜中重新分析单细胞RNA测序数据.
- 在口腔角质细胞中检测细菌大分子 (rRNA,mRNA,甘油脂) 使用in situ杂交和免疫组织化学.
- 在体外和体内实验中挑战细胞与细菌成分并分析epikine表达的实验.
- 应用Drug2Cell算法用于药物疗效预测.
主要成果:
- 在口腔上皮细胞和脑膜细胞中发现了多菌特征.
- 在人类口腔角质细胞内检测到持久性细菌大分子 (PIMs).
- 较高的PIM度与增强的受体-连接体信号传递和epikine表达相关.
- 内源性利波泰荷酸,而不是脂聚糖,直接影响了epikine的表达.
- PIM检测改变了药物的疗效预测.
结论:
- 多细菌细胞内宏分子 (PIMs) 存在于粘膜上皮细胞中.
- PIMs线性驱动单细胞效应因子细胞因子表达.
- PIMs影响药物反应,影响治疗策略.
- 了解PIM对于理解单细胞水平上健康和疾病的宿主微生物动态至关重要.
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