极化异位细胞和离子脂物种涉及到启动克拉斯林介导的内分细胞
bioRxiv : the preprint server for biology
|October 17, 2024
概括
发芽酵母使用Snc1/2蛋白和离子脂来招募Yap1801/2,一个关键蛋白,到子细胞中. 这种机制将两极化克拉中介性内分泌 (CME) 与细胞生长过程中的两极化分泌联系起来.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 克拉特林介导内细胞分裂 (CME) 对细胞过程至关重要,但其启动机制仍然不完全理解.
- 内细胞适配蛋白Yap1801/Yap1802 (酵母AP180) 在极化生长过程中,对于发芽的酵母子细胞中CME启动至关重要.
- 对Yap1801/2在子细胞等离子体膜中的优先招募并未得到充分解释.
研究的目的:
- 为了研究芽酵母中Snc1/2蛋白的时空动力学.
- 为了阐明Yap1801/2被招募到子细胞的机制.
- 为了建立两极分泌和两极化CME之间的联系.
主要方法:
- 使用功能性,光标记的Snc1/2从内源的位置表达出来.
- 分析了Snc1/2在活芽酵母细胞中的时空动态.
- 研究了离子脂在Snc1/2-介导招募中的作用.
主要成果:
- 发现Snc1/2蛋白质可以优先地招募Yap1801/2到正在生长的子细胞的血膜中.
- 这种招募是由Snc1/2和离子脂的联合存在促进的.
- 有证据表明,Snc1/2对于极化生长所必需的外细胞囊泡起到v-SNARES的作用.
结论:
- 阴离子脂和Snc1/2蛋白的巧合驱动了Yap1801/2的招募,在生长中的子细胞中启动了CME.
- 这项研究通过Snc1/2-Yap1801/2相互作用直接将两极化CME与两极化分泌物联系起来.
- 这些发现提供了对CME如何与芽酵母的极化生长协调的机制的理解.
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