探索药用植物的抗菌活性和与多西环素对细菌物种的协同效应
Joel Frank Kenmeni1, Ibrahim Sifi2, Borel Ndezo Bisso1
1Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
TheScientificWorldJournal
|October 17, 2024
概括
药用植物提取物显示抗生素对抗细菌菌株的抗生素潜力. 结合多西环素的Solanum torvum提取物有效抑制和根除生物膜,这表明新的结合抗菌疗法.
科学领域:
- 药理学和植物化学
- 微生物学与传染病的研究
背景情况:
- 药用植物是具有抗微生物特性的生物活性化合物的公认来源.
- 抗微生物耐药性需要探索新的治疗策略,包括植物衍生化合物.
- 细菌生物膜在临床环境中带来了重大挑战,因为它们对常规抗生素具有强硬性.
研究的目的:
- 评估来自九种药用植物的甲醇和乙醇提取物对抗细菌菌株的抗菌膜活性.
- 为了研究这些植物提取物与多克西环素结合的协同效应.
- 为了确定植物化学特征,并量化提取物中和黄的总含量.
主要方法:
- 植物化学查,确定总和黄含量.
- 苏微稀释用于最低抑制度 (MIC) 和微板测定生物膜形成.
- 3-(4,5-二甲基-2-thiazolyl) -2,5-二-2-H-四-化物 (MTT) 测定抗菌膜活性和协同作用研究的棋盘方法.
主要成果:
- 所有植物提取物都含有,黄,类固醇,三烯和.
- 乙醇提取物Eucalyptus globulus和Psidium guajava,以及甲醇的Syzygium jambos提取物显示出针对Bacillus anthracis的显著抗菌活性 (MIC 64μg/mL).
- 索兰 (Solanum torvum) 乙醇提取物与多西环素显示出协同作用的抗生物膜作用,在经过测试的Bacillus菌株中抑制和消灭生物膜.
结论:
- 药用植物提取物具有显著的抗菌和抗菌膜特性.
- 结合Solanum torvum乙醇提取物与多西环素的组合显示,作为一种针对Bacillus生物膜的新型治疗方法具有前景.
- 对Solanum torvum的进一步研究可能会导致新组合抗菌疗法的开发.
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