口腔细菌计数器使用介电阻测量:有用性和使用考虑因素
Akira Imakiire1, Sakiko Soutome1, Yuichi Nakamura1
1Department of Oral Health, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JPN.
Cureus
|October 17, 2024
概括
口腔细菌计数器可能会因为消毒剂和电解质而高估细菌计数,并且在粘度增加时低估它们. 精确的口腔细菌量化需要考虑这些因素,并使用像DR-PCR这样的补充方法.
科学领域:
- 微生物学 微生物学
- 生物医学工程 生物医学工程
- 牙科诊断 牙科诊断 牙科诊断
背景情况:
- 口腔中含有与全身性疾病相关的细菌.
- 口腔细菌计数器 (PHC公司) 提供快速的细菌计数,使用介电电阻测量.
- 一个限制是它在检测无生命力的细菌方面的潜力.
研究的目的:
- 评估消毒剂,电解质和粘度对口腔细菌计数器准确性的影响.
- 为了确定该设备是否与可行的细菌相似地量化非可行的细菌.
主要方法:
- 使用口腔细菌计数器评估消毒剂 (povidone-iodine,乙化物,chlorexidine) 和盐水.
- 通过将无菌水与甘油混合来评估粘度效应.
- 对比口腔细菌计数器的结果与延迟的实时聚合酶链反应 (DR-PCR) 和培养方法来定量可活性的细菌.
主要成果:
- 使用消毒剂 (5%CHX,7%PV-I) 观察到高读数,即使没有活性的细菌.
- 糖醇的粘度增加导致细菌数量减少.
- 电解质,特别是盐水,导致读数更高,该设备检测了可活和不可活的细菌.
结论:
- 消毒剂,粘度和电解质可以影响口腔细菌计数器的准确性.
- 建议将口腔细菌计数器数据与DR-PCR等方法结合起来,以进行可靠的口腔细菌评估.
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