不同形式的化肥的应用会影响土壤细菌群体,但不会影响核心ARG配置
Ruiqiang You1,2, Yang Yu1, Min Shen1
1Jiangsu Key Laboratory for Bioresources of Saline Soils, Yancheng Teachers University, Yancheng, Jiangsu, China.
Frontiers in microbiology
|October 17, 2024
概括
化学肥对土壤抗生素耐药性基因 (ARG) 的影响很小. 酸肥 (ON) 类似于对照水平,而 (AN) 和酸盐 (NN) 显示波动. 细菌群落受到影响,但ARG增殖风险与脱化有关.
科学领域:
- 环境微生物学 环境微生物学
- 土壤科学 土壤科学
- 遗传学和基因组学 在
背景情况:
- 化学化肥在农业中被广泛使用,引发了人们对它们对土壤微生物群落和抗生素耐药性基因 (ARG) 扩散的潜在影响的担忧.
- 了解化肥应用与ARG丰富性之间的关系对于评估环境风险和开发可持续农业实践至关重要.
研究的目的:
- 研究不同化学化肥 (,酸盐,尿素) 对土壤中ARG的形状和丰度的影响.
- 在各种肥条件下评估与循环相关的基因 (NCRG) 和ARG之间的相关性.
主要方法:
- 进行了为期30天的微观实验,使用了四种治疗方法:对照 (CK), (AN),酸 (NN) 和尿素 (ON).
- 使用元基因组测序来量化核心ARG和NCRG的丰度.
- 使用主要成分分析和聚类分析来评估对ARG概况和细菌群落的影响.
主要成果:
- 化学化肥对土壤中核心ARG概况的整体影响很小.
- 尿素 (ON) 治疗显示ARG丰度与对照组 (CK) 类似,而 (AN) 和酸盐 (NN) 治疗显示时间波动.
- 在不同的肥处理中观察到细菌群体的明显变化,但由于未受影响的潜在宿主,对核心ARG的影响是有限的.
结论:
- 化学化肥的应用对土壤中ARG的丰富程度的影响有限,减轻了对ARG积累的担忧.
- 环境友好的化肥,如可控释放尿素配方,可能更好.
- NCRG (参与固和脱) 和ARG之间的正相关性表明,在强烈的脱过程中存在ARG扩散的潜在风险.
更多相关视频
09:49Divergence of Root Microbiota in Different Habitats based on Weighted Correlation Networks
Published on: September 25, 2021
4.3K
08:58Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
6.3K
相关概念视频
Overview of Nitrogen Metabolism
7.8K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of ammonia, ammonium ions, nitrate, nitrite, or nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
7.8K
The Roles of Bacteria and Fungi in Plant Nutrition
35.1K
Plants have the impressive ability to create their own food through photosynthesis. However, plants often require assistance from organisms in the soil to acquire the nutrients they need to function correctly. Both bacteria and fungi have evolved symbiotic relationships with plants that help the species to thrive in a wide variety of environments.
35.1K
The Nitrogen Cycle
51.7K
Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...
51.7K
Metabolism of Chemolithotrophs
2
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
2
Inorganic Nitrogen Assimilation
5
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
5
Bacterial Phylum Actinobacteria
Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
