Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Vertebral Fractures Beyond Bone Density in Breast Cancer: A Real-World Study of Endocrine Therapy and FRAX Reclassification.

Journal of clinical medicine·2026
Same author

Cardiovascular disease mortality attributable to monthly non-optimal temperature in the united states: a county-level analysis.

American journal of preventive cardiology·2026
Same author

Prevalence of somatic SF3B1R625H mutation in lactotroph tumours from a multi-centric cohort: a digital PCR-based study.

European journal of endocrinology·2026
Same author

Acute Effects of Different Training Sessions on the Extended Blood Steroid Profile in Elite Male Football Players.

Sports medicine (Auckland, N.Z.)·2026
Same author

Determinants of Quality of Life in Patients with Immune-Checkpoint-Inhibitor Induced Secondary Adrenal Insufficiency.

The Journal of clinical endocrinology and metabolism·2026
Same author

Preoperative Very-Low-Calorie Ketogenic Diet Versus Low-Calorie Diet in Bariatric Surgery: A Prospective Comparative Study.

Nutrients·2026

相关实验视频

Updated: May 9, 2026

A Novel Method: Super-selective Adrenal Venous Sampling
06:08

A Novel Method: Super-selective Adrenal Venous Sampling

Published on: September 15, 2017

23.3K

循环miRNAs和机器学习用于横向化初级阿尔多斯特罗尼主义.

Bálint Vékony1,2, Gábor Nyirő1,2,3, Zoltan Herold2

  • 1Department of Endocrinology (B.V., G.N., N.S., B.K.S., P.I.), Faculty of Medicine, Semmelweis University, Budapest, Hungary.

Hypertension (Dallas, Tex. : 1979)
|October 17, 2024
PubMed
概括

循环的microRNAs (miRNAs) 和机器学习可以准确地区分单边的和双边的初级阿尔多斯特隆主义. 这种微侵袭性方法有助于早期诊断和治疗高血压,绕过侵袭性上腺静脉采样.

关键词:
深度学习是一种深度学习.过高阿尔多斯特主义这种高血压,高血压.微RNAs 是一个微型RNA.

更多相关视频

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

8.1K
mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

2.5K

相关实验视频

Last Updated: May 9, 2026

A Novel Method: Super-selective Adrenal Venous Sampling
06:08

A Novel Method: Super-selective Adrenal Venous Sampling

Published on: September 15, 2017

23.3K
Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

8.1K
mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

2.5K

科学领域:

  • 内分泌学 在内分泌学.
  • 基因组学就是基因组学.
  • 计算生物学 计算生物学

背景情况:

  • 主要的阿尔多斯特是二次高血压的重要原因,需要准确地区分单边和双边形式以进行适当的治疗.
  • 上腺静脉采样,目前的黄金标准,由于侵入性和解释困难,提出了挑战.
  • 探索像循环微RNA (miRNAs) 这样的微侵入性生物标志物对于改善诊断策略至关重要.

研究的目的:

  • 调查循环miRNAs与机器学习相结合在区分单边和双边初级阿尔多斯特隆主义中的有效性.
  • 评估使用外周血液样本进行miRNA分析的可行性,减少侵入性手术的需要.
  • 开发一个预测模型,准确地分类初级阿尔多斯特主义.

主要方法:

  • 血miRNA分析是在使用Illumina MiSeq.对原发性阿尔多斯铁症患者进行的.
  • 生物信息学和机器学习确定了通过定量PCR验证的候选miRNA.
  • 使用深度学习模型进行分类,使用上腺静脉采样和外围样本.

主要成果:

  • 一个6-miRNA模型实现了87.1%的曲线下面积 (AUC),以区分单边的初级代体.
  • 深度学习模型表现出高准确性,在一个子集上达到100%,在108名患者的全部队列上达到86.7%的AUC.
  • 外围样本被证明适合用于miRNA分析,证实了一种不那么侵入性的诊断方法的潜力.

结论:

  • 循环miRNAs的机器学习分析提供了一个有前途的,最小侵入性的替代方案,用于初级aldosteronism横向化.
  • 这种方法可以加快双边上腺增生症的治疗启动,因为它可以在没有进一步定位的情况下进行早期识别.
  • 利用循环的miRNA为患者和医疗保健提供者在治疗二次高血压方面提供了显著的好处.