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循环miRNAs和机器学习用于横向化初级阿尔多斯特罗尼主义
Bálint Vékony1,2, Gábor Nyirő1,2,3, Zoltan Herold2
1Department of Endocrinology (B.V., G.N., N.S., B.K.S., P.I.), Faculty of Medicine, Semmelweis University, Budapest, Hungary.
Hypertension (Dallas, Tex. : 1979)
|October 17, 2024
概括
循环的microRNAs (miRNAs) 和机器学习可以准确地区分单边的和双边的初级阿尔多斯特隆主义. 这种微侵袭性方法有助于早期诊断和治疗高血压,绕过侵袭性上腺静脉采样.
科学领域:
- 内分泌学 在内分泌学.
- 基因组学就是基因组学.
- 计算生物学 计算生物学
背景情况:
- 主要的阿尔多斯特是二次高血压的重要原因,需要准确地区分单边和双边形式以进行适当的治疗.
- 上腺静脉采样,目前的黄金标准,由于侵入性和解释困难,提出了挑战.
- 探索像循环微RNA (miRNAs) 这样的微侵入性生物标志物对于改善诊断策略至关重要.
研究的目的:
- 调查循环miRNAs与机器学习相结合在区分单边和双边初级阿尔多斯特隆主义中的有效性.
- 评估使用外周血液样本进行miRNA分析的可行性,减少侵入性手术的需要.
- 开发一个预测模型,准确地分类初级阿尔多斯特主义.
主要方法:
- 血miRNA分析是在使用Illumina MiSeq.对原发性阿尔多斯铁症患者进行的.
- 生物信息学和机器学习确定了通过定量PCR验证的候选miRNA.
- 使用深度学习模型进行分类,使用上腺静脉采样和外围样本.
主要成果:
- 一个6-miRNA模型实现了87.1%的曲线下面积 (AUC),以区分单边的初级代体.
- 深度学习模型表现出高准确性,在一个子集上达到100%,在108名患者的全部队列上达到86.7%的AUC.
- 外围样本被证明适合用于miRNA分析,证实了一种不那么侵入性的诊断方法的潜力.
结论:
- 循环miRNAs的机器学习分析提供了一个有前途的,最小侵入性的替代方案,用于初级aldosteronism横向化.
- 这种方法可以加快双边上腺增生症的治疗启动,因为它可以在没有进一步定位的情况下进行早期识别.
- 利用循环的miRNA为患者和医疗保健提供者在治疗二次高血压方面提供了显著的好处.
相关概念视频
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
02:30siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
01:25Nucleic Acid Structure
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
DNA Structure
DNA has a double-helix structure. The...