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相关概念视频

RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

18.8K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.8K
Euchromatin01:01

Euchromatin

6.8K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
6.8K
Heterochromatin02:38

Heterochromatin

11.5K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
11.5K

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相关实验视频

Updated: Jun 10, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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SpatialRef:一个带有已知点注释的空间omics的引用.

Ting Cui1,2,3, Yan-Yu Li1,2,4,5,3,6, Bing-Long Li1,2,3

  • 1The First Affiliated Hospital & National Health Commission Key Laboratory of Birth Defect Research and Prevention, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China.

Nucleic acids research
|October 17, 2024
PubMed
概括

SpatialRef是一个新的数据库,手动策划的空间omics数据. 它在多种物种和组织类型中提供高质量的注释点标签,用于先进的空间奥米克分析.

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Use of Single Molecule Fluorescent In Situ Hybridization SM-FISH to Quantify and Localize mRNAs in Murine Oocytes
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Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
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相关实验视频

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Use of Single Molecule Fluorescent In Situ Hybridization SM-FISH to Quantify and Localize mRNAs in Murine Oocytes
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科学领域:

  • 空间生物学和基因组学
  • 生物信息学和数据科学
  • 分子和系统生物学分子和系统生物学

背景情况:

  • 空间奥米克技术可以生成组织的详细分子地图,但准确的点位注释是具有挑战性的.
  • 了解空间组织对于破译组织功能和生物过程至关重要.
  • 现有的数据集缺乏对各种空间学研究的全面,手动策划的注释.

研究的目的:

  • 开发SpatialRef,一个手动策划的空间omics数据数据库,具有可靠的点位标签.
  • 为分析空间转录组学和其他空间组学数据的研究人员提供高质量的资源.
  • 通过提供跨多种物种和技术的标准化注释来促进下游分析.

主要方法:

  • 从>17种组织类型的>900万个斑点在人类,老鼠和Drosophila中汇集和手动策划.
  • 通过广泛的审查和严格的质量控制协议来确保数据质量.
  • 来自多种空间测序技术和>400个点/域类型的综合数据.

主要成果:

  • SpatialRef提供了全面的,高质量的空间奥米克数据与已知的点标签.
  • 该数据库涵盖了各种物种,组织类型和空间奥米克技术.
  • 它支持各种下游分析,包括差异基因表达,途径注释和空间通信.

结论:

  • SpatialRef 是一个有价值的资源,用于推进空间奥米克研究.
  • 该数据库简化了注释过程,提高了空间奥米克分析的可靠性.
  • 它有助于理解组织组织,空间领域的功能相关性和潜在的生物效应.