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来自侧向双臂神经核的多个神经网络调节类似咳的行为,并将咳与疼痛协调
Mingtong Lin1, Mingzhe Liu1, Chuqin Huang1
1National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China; and.
American journal of respiratory cell and molecular biology
|October 17, 2024
概括
侧侧核 (LPBN) 调节咳的敏感性和疼痛. 在LPBN中的特定神经元通过不同的机制协调疼痛和咳.
科学领域:
- 神经科学是一个神经科学.
- 呼吸系统生理学 呼吸系统生理学
背景情况:
- 实验性疼痛可以减少咳的敏感性.
- 众所周知,侧侧核 (LPBN) 能够整合疼痛和呼吸信号.
- 对于LPBN在咳调节和疼痛诱导咳调节中的作用尚不清楚.
研究的目的:
- 为了研究LPBN中GABAergic和glutamatergic神经元的功能.
- 确定这些神经元如何调节小鼠的咳敏感性和与疼痛相关的咳变化.
- 探索LPBN在协调疼痛和咳行为中的作用.
主要方法:
- 在小鼠中利用化学遗传方法来操纵特定的神经元群体.
- 采用腺相关病毒 (AAV) 追踪来映射从LPBN到周水管灰色的神经元投射.
- 在神经元激活或抑制后评估咳敏感性和类似咳的行为.
主要成果:
- 在咳挑战期间,LPBN神经元被激活.
- 对LPBN神经元的非特异性抑制抑制了类似咳的行为.
- 抑制LPBNGABAergic神经元降低了咳的敏感性.
- 抑制LPBN谷氨酸性神经元,包括那些投射到水管灰度的神经元,抵消了疼痛引起的咳敏感度的降低.
结论:
- 在LPBN中的GABAergic和glutamatergic神经元对于调节咳敏感性至关重要.
- 这些神经元群体通过抑制和激活通路协调疼痛和咳.
- 该LPBN作为一个关键的中脑枢纽,用于整合疼痛和咳.
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