解读Aflatoxin B1影响控制癌症的关键分子途径:使用CTD和PANTHER数据库的生物信息学研究
Ankita Kapri1, Dheer Singh1, Suneel Kumar Onteru2
1Molecular Endocrinology, Functional Genomics & Systems Biology Laboratory, Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal, 132001, Haryana, India.
阿弗拉托克素B1 (AFB1) 暴露会影响肺癌,结肠直肠癌,肝癌,胃癌和乳腺癌中的关键分子通路. 这项研究确定了AFB1诱导癌症的受影响途径和13个潜在生物标志物基因.
科学领域:
- 毒理学 毒理学 毒理学
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 甲素B1 (AFB1) 是一种在食品中发现的强有力的致癌性真菌毒素.
- 暴露于AFB1与严重的健康问题有关,包括肝毒性和各种癌症.
- 在癌症发育过程中受到AFB1影响的分子通路尚不清楚.
研究的目的:
- 在五种主要癌症类型中,通过AFB1暴露显著改变的分子通路的识别.
- 确定那些可以作为AFB1诱导癌症生物标志物的关键基因.
- 提供关于AFB1在癌症发病和进展中的作用的见解.
主要方法:
- 从肺癌,结肠直肠癌,肝癌,胃癌和乳腺癌的比较毒基因组学数据库 (CTD) 中选择的AFB1响应基因.
- 利用PANTHER数据库进行统计过度代表性测试,以确定受影响的途径.
- 在已识别的途径上进行基因分析,以找到潜在的生物标记物基因.
主要成果:
- 确定了淋巴激素释放激素受体 (GnRHR),CCKR信号传递和血管生成作为多种癌症中显著受影响的途径.
- 在肝癌和胃癌中,AFB1对亡和Wnt信号通路产生了影响.
- 在结直肠癌中,Wnt,CCKR和GnRHR通路受到影响最大.
- 包括FOS和AKT1在内的13个关键基因被确定为潜在的生物标志物.
结论:
- 在多种癌症类型中,AFB1显著影响涉及癌症发展的关键分子通路.
- 鉴定到的关键基因可以作为AFB1诱导癌症和体外毒理学研究的有价值的生物标志物.
- 对这些途径和基因的进一步研究可以阐明AFB1的致癌机制.
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