(E) -β-Farnesene 通过抑制其有益的肠道微生物,保护大米免受大米条纹干虫的攻击.
Lei Yang1,2,3, Pei-Tao Zhang1, Miao-Miao Li1,2
1Laboratory for Conservation and Use of Important Biological Resources of Anhui Province, Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, College of Life Sciences, Anhui Normal University, Wuhu 241002, China.
Journal of agricultural and food chemistry
|October 17, 2024
概括
米植物产生一种叫做 (E) -β-farnesene (Eβf) 的化合物,该化合物会损害米条纹茎 (SSB) 的肠道微生物. 这种破坏损害了昆虫的发展和生存,揭示了一个新的植物防御策略.
科学领域:
- 植物与昆虫的相互作用
- 微生物生态学 微生物生态学
- 生物化学 生物化学
背景情况:
- 草食肠道微生物对于发育,营养和生存至关重要.
- 植物的防御系统可以准这些微生物,但这在米中还没有得到充分的研究.
- 米条纹干 (SSB) 是重要的农业害虫.
研究的目的:
- 调查米合成酶基因Os04g0341500在植物对SSB的防御中的作用.
- 要确定合成的化合物 (E) -β-法尔内森 (Eβf) 是否影响SSB发育和肠道微生物群.
- 阐明Eβf影响SSB的机制.
主要方法:
- 鉴定和表征了大米烯合成酶基因Os04g0341500.
- 使用人工饮食评估了Eβf对SSB幼虫发育和生存的影响.
- 分析了SSB肠道微生物群落的变化,使用高通量安普利康测序.
- 进行了体外实验,以确认Eβf对肠道微生物的抗菌活性.
主要成果:
- 米基因Os04g0341500是由SSB养诱导的,并合成了 (E) -β-法纳 (Eβf).
- Eβf显著损害了SSB幼虫的发育和生存.
- 与对照组相比,SSB养的Eβf显示有益的肠道微生物减少.
- 在实验室中,Eβf直接抑制了SSB肠道微生物的生长.
结论:
- 米植物可以通过生产Eβf来保护自己,这会破坏SSB.的肠道微生物群.
- 由于Eβf减少有益的肠道微生物,会对SSB幼虫的发育和生存产生负面影响.
- 这项研究强调了一种新的植物防御机制,涉及对昆虫肠道微生物群落的操纵.
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