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Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Updated: Jun 21, 2026

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
09:44

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

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在基因代码操纵方面达到新的高度,使用高通量查.

Briana R Lino1, Sean J Williams1, Michelle E Castor1

  • 1Chemical and Biological Engineering Department, Tufts University, Medford, Massachusetts 02155, United States.

Chemical reviews
|October 17, 2024
PubMed
概括

遗传密码的扩展使得非正规氨基酸 (ncAAs) 可以被纳入蛋白质中,从而增强其功能. 高通量选加速了新型ncAA含有蛋白质的发现,具有多种应用.

科学领域:

  • 生物化学 生化学
  • 合成生物学 合成生物学
  • 蛋白质工程是指蛋白质工程.

背景情况:

  • 20种正规的氨基酸限制了蛋白质的化学和物理特性.
  • 基因编码操纵允许引入非正规氨基酸 (ncAAs) 来增强或改变蛋白质的功能.

研究的目的:

  • 审查将ncAAs引入生物合成蛋白质的策略的进展.
  • 突出高通量选在这些进展中的作用.

主要方法:

  • 通过新的选择方法对氨基酸-tRNA合成酶 (aaRSs) 和tRNA进行工程.
  • 改进蛋白质翻译机制和替代遗传代码的细胞组件.
  • 利用发现平台对含有ncAA的蛋白质进行高通量选.

主要成果:

  • 新的选择方法提高了ncAA纳入效率和选择性.
  • 工程细胞组件增强了ncAA的整合.
  • 发现平台产生具有增强功能的创新的结合和酶.
  • 进步已经导致有希望的药物线索和生物催化剂.

结论:

  • 高通量方法通过发现意想不到的功能来显著影响ncAA的整合和应用.

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  • 实验技术,计算蛋白质设计和机器学习方面的未来创新将进一步推进该领域.