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Types Of Column Chromatography01:29

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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
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Affinity Chromatography01:03

Affinity Chromatography

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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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集成的多维色谱在准备规模,用于净化寡核酸的净化.

Chiara Nosengo1, Desiree Bozza1, Giulio Lievore2

  • 1Department of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, via L. Borsari 46, Ferrara 44121, Italy.

Journal of chromatography. A
|October 17, 2024
PubMed
概括

一个集成批量处理的寡核酸净化过程结合了两个染色学步骤,减少了生产时间,提高了可重复性. 这种自动化方法提高了治疗性核酸制造的纯度和产量.

关键词:
药品中的活性成分 药品中的活性成分阳离子交换色谱学 阳离子交换色谱学多维色谱是多维的色谱.有氧核酸类的准备性色谱学 准备性色谱学反向相位染色学反向相位染色学

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科学领域:

  • 制药科学 制药科学
  • 生物技术是生物技术.
  • 分析化学 分析化学

背景情况:

  • 治疗性寡核酸为疾病治疗提供了特定的基因表达调节.
  • 高纯度的活性药物成分 (API) 对于寡核酸治疗药物至关重要,需要进行密集的净化.
  • 准备性液体染色学,特别是阴离子交换 (AEX) 和离子对逆相 (IP-RP) 染色学,是大规模寡核酸净化标准.

研究的目的:

  • 评估一个"集成批量工艺",以 (半) 准备性规模净化单链DNA寡核酸.
  • 为了比较综合方法与传统单批染色学运行的性能.
  • 证明多维和正交色谱对于增强的寡核酸净化的潜力.

主要方法:

  • 实施"集成批量工艺",结合两个连续的染色学步骤 (AEX和IP-RP),没有中间持有或采样.
  • 使用综合方法对单链DNA寡核酸的净化.
  • 性能参数 (纯度,产量,时间) 与在各种实验条件下的传统单批运行进行比较.

主要成果:

  • 集成批处理过程表明了对寡核酸净化有很大的潜力.
  • 这种方法提供了与连续单批净化相比,总体生产时间的缩短.
  • 由于高度自动化,集成方法提高了净化过程的稳定性和可重复性.

结论:

  • "集成批量工艺"为治疗性寡核酸的下游加工提供了一个可行和高效的战略.
  • 这种自动化,多维色谱方法解决了单步净化中固有的纯度-产量权衡问题.
  • 该研究强调了整合直角色谱技术的可行性,以简化和改进基于寡核酸的治疗药物的制造.