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Can Yang¹, Jinwei Gao¹, Hao Wu¹

¹Hunan Fisheries Science Institute, Changsha, 410153, China.

Fish & shellfish immunology

|October 17, 2024

概括

黑 TBK1 (bcTBK1) 和黑 IRF5 (bcIRF5) 在抗病毒防御中起着关键作用. 这项研究揭示了bcIRF5通过酸化激活bcTBK1,促进GCRV感染细胞中的PANoptosis,并提高鱼类的生存率.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
病毒学病毒学

背景情况:

坦克结合激酶1 (TBK1) 是干扰素抗病毒信号的一个关键因素.
之前的研究表明,黑鱼TBK1 (bcTBK1) 和黑鱼IRF5 (bcIRF5) 在感染GCRV的细胞中促进细胞死亡.

研究的目的:

为了研究bcTBK1和bcIRF5在黑鱼中的单独和联合功能.
阐明bcIRF5在GCRV感染期间影响bcTBK1活动的机制.
了解它们在抗病毒反应和细胞死亡途径中的作用.

主要方法:

进行了体内和体外实验.
进行了基因表达分析 (RIPK1,caspase1,caspase3,bax,IL1β的mRNA水平).
使用过度表达研究,血素-素 (HE) 染色,道染色和酸化试验.

主要成果:

在各种组织中,bcTBK1和bcIRF5表达因GCRV感染而调节.
在GCRV感染后,bcTBK1和bcIRF5的协同过度表达上调了亲细胞亡基因和增强的caspase3活性.
bcIRF5促进了bcTBK1的酸化,导致PANoptosis的增加和黑鱼生存率的提高.

结论:

bcIRF5通过酸化激活bcTBK1,促进GCRV感染细胞中的PANoptosis.
bcTBK1-bcIRF5相互作用对于黑鱼的抗病毒防御和细胞死亡调节至关重要.
这一途径可以提高病毒感染期间宿主生存率.

关键词:

抗病毒免疫抗病毒免疫在GCRV中使用GCRV.在IRF5中,IRF5是指IRF5.这就是PANoptosis.在TBK1中,

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