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相关实验视频

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A Small Volume Procedure for Viral Concentration from Water
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在活性污泥中对病毒的方法评估:度,测序和识别.

Yulin Zhang1, Xiawan Zheng1, Weifu Yan1

  • 1Environmental Microbiome Engineering and Biotechnology Lab, Department of Civil Engineering, The University of Hong Kong, Pokfulam, Road, Hong Kong, China.

The Science of the total environment
|October 17, 2024
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概括

这项研究优化了在活性污泥 (AS) 中分析病毒的方法,建议化,过和再悬浮 (FFR) 和接触流过用于度. geNomad和长读测序是最好的病毒识别在AS.

关键词:
活性污泥活动污泥flokculation 过和复悬浮的使用方法菌体是一种菌体.接触式流量过的过方法病毒 病毒 病毒废水处理厂 废水处理厂

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科学领域:

  • 环境微生物学环境微生物学
  • 病毒学 病毒学
  • 污水处理 污水处理 污水处理

背景情况:

  • 活性污泥 (AS) 是废水处理中的关键微生物生态系统,它容纳了大量的病毒.
  • 由于集中,提取和测序方面的挑战,AS中的病毒群体未得到充分研究.
  • 了解AS病毒对于优化废水处理过程至关重要.

研究的目的:

  • 评估和比较在活性污泥中进行病毒分析的不同方法.
  • 为了确定AS病毒组研究的最佳度技术,测序方法和生物信息工具.
  • 为AS生态系统中的病毒组成和宿主相互作用提供见解.

主要方法:

  • 四种病毒度方法的比较:花,过,复悬浮 (FFR) 和超离心.
  • 评估两种测序方法:短读和长读测序.
  • 评估四种病毒识别生物信息工具,重点是geNomad.

主要成果:

  • FFR产生了最长的病毒DNA,而超离心提供了最高的DNA产量.
  • 建议使用FFR和触流过 (100kDa) 来集中来自大量AS的病毒.
  • geNomad和长读测序显示了病毒识别和汇集统计数据的卓越性能.
  • 为了获得全面的病毒目录,需要多种度方法.
  • 亚氏病病毒组由菌体 (95.1%) 主导,主要是Caudovirales,有毒菌体向Pseudomonadota.

结论:

  • 这项研究提供了有效的活性污泥病毒研究的方法框架.
  • 推的方法包括FFR或触流过用于度和geNomad,用于识别的长读序列.
  • 这些发现凸显了AS和其主要宿主中毒菌体的主导地位.