澳大利亚白菌保护Gloriosa superba L.免受Alternaria alternata的感染
Pradeep Semwal1,2, Shashank Kumar Mishra1, Basudev Majhi1,2
1Microbial Technologies Division, Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India.
World journal of microbiology & biotechnology
|October 17, 2024
概括
澳大利亚白菌内菌 (NBRI GS34) 有效地对抗Gloriosa superba.中的Alternaria alternata叶病. 这种有益的微生物增强了植物生长,并增强了有价值的药用化合物,提供了可持续的杀菌剂替代品.
科学领域:
- 植物病理学 植物病理学
- 微生物学 微生物学
- 药理学是指药理学,即药理学是指药理学.
背景情况:
- 格洛里奥萨 (Gloriosa superba L.) 是一种医学上至关重要的植物物种.
- 由Alternaria alternata引起的叶病显著降低了Gloriosa superba的生产力.
- 开发有效的疾病管理策略对于这种有价值的植物至关重要.
研究的目的:
- 调查澳大利亚白菌内菌 (NBRI GS34) 对Alternaria alternata叶病的保护潜力.
- 评估NBRI GS34对Gloriosa superba生长和生物活性代谢物产生的影响.
- 探索NBRI GS34和Alternaria alternata之间相互作用的基础机制.
主要方法:
- 与A. alternata一起共同培养NBRI GS34,以评估酶活性 (蛋白酶,基因酶).
- 扫描电子显微镜 (SEM) 和气色谱-质谱 (GC-MS) 用于分析抗真菌化合物.
- 温室实验用于评估疾病发生率,植物生长和防御基因表达.
- 高性能液体染色学 (HPLC) 用于量化关键的生物活性代谢物 (素,素).
主要成果:
- 在温室试验中,NBRI GS34注射显著减少了78%的叶病发病率.
- 同培养增强了蛋白酶 (30-50%) 和胆酶 (6-28%) 的活性,表明了协同作用.
- 用NBRI GS34治疗促进了植物生长,并增加了光素 (52%) 和素 (33%) 的度.
- 鉴定出了抗真菌化合物,如安德卡诺酸和碳酸.
- 观察到与植物防御有关的基因的升级.
结论:
- 澳大利亚白菌内菌 (NBRI GS34) 显示出作为生物控制剂对抗Gloriosa superba叶病的显著潜力.
- NBRI GS34增强了植物生长,并增加了重要的药用化合物的度.
- 这种内生植物代表了化学杀菌剂的可持续替代品,具有有前途的制药应用.
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