相关实验视频

Updated: Jun 13, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

平行基因组规模的CRISPR-Cas9屏幕将人类多能干细胞的身份与健康相比分开

Bess P Rosen^1,2, Qing V Li^1,3,4, Hyein S Cho¹

¹Developmental Biology Program, Sloan Kettering Institute, New York, NY, USA.

Nature communications

|October 17, 2024

概括

人类多能干细胞 (hPSCs) 具有自我更新和分化能力. 这项研究使用了CRISPR屏幕来识别调节hPSC适应性和身份的基因,揭示了多能性的关键调节者.

科学领域:

干细胞生物学干细胞生物学
基因组学就是基因组学.
分子和细胞生物学分子和细胞生物学

背景情况:

多能干细胞 (PSC) 具有自我更新和分化潜力.
了解干细胞适应性和身份的调节对于再生医学至关重要.

研究的目的:

研究人类PSC (hPSC) 中的自我更新 (健康) 和多能身份之间的相互作用.
在早期分化过程中识别调节干细胞适应性和多能特性的基因.

主要方法:

在hPSC中进行了基因组规模的CRISPR-Cas9功能丧失查.
使用比较分析来区分与干细胞适应性和多能性身份相关的基因功能.

主要成果:

确定了线粒体和新陈代谢调节器在干细胞适应性中的独特作用.
在分化过程中确定了关键的染色质调节剂,以保持多能特性的身份.
发现了一组核心基因,包括调节身体健康和身份的染色体因子.

结论:

公正的查解开了干细胞适应性和多能身份的相互关联方面.
为研究多能性调节提供了宝贵的资源.
为分类干细胞中的基因功能提供了一个框架.

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Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

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CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions

CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions

Published on: November 1, 2024

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CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...