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从Benincasa hispida var. 中降解的多糖. chieh-qua 如何:揭示降解化合物的生物活性特性

Xin-Yuan Sheng¹, Hai-Jiang Zhang², Xin-Juan Chen³

¹School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, P. R. China.

Chemistry & biodiversity

|October 17, 2024

概括

使用过氧化-酸氧化氧化的降解-多糖化合物增强了它们的抗氧化和抗铁酶活性. 这种优化的方法显示了食品和医疗应用的潜力.

科学领域:

食品科学食品科学食品科学
生物化学生物化学
聚合物化学聚合物化学

背景情况:

清水多糖 (BHCP) 是具有潜在生物活性的天然聚合物.
修改多糖可以改变它们的特性和增强功能.
氧化降解提供了一种修改多糖结构的方法.

研究的目的:

开发和优化一种有效的降解水多糖 (BHCP) 的方法.
评估降解对多糖特性的影响,包括分子量和糖含量.
评估降解多糖的抗氧化和抗铁酶活性.

主要方法:

使用Box-Behnken响应表面设计优化降解条件.
过氧化-甲酸 (H2O2-VC) 氧化系统用于多糖的降解.
降解多糖的净化和结构分析 (DBHCP-1,DBHCP-2).
在体外测试以确定抗氧化剂和抗铁酶活性.

主要成果:

优化的降解条件:19.5mM H2O2-VC,46.4°C,1.0小时.
降解降低了BHCP的平均分子量,并增加了BHCP的总糖含量.
与原生BHCP相比,纯化降解多糖 (DBHCP和DBHCP-1) 显示出增强的抗氧化和抗铁酶活性.

结论:

H2O2-VC系统提供了一种有效的方法来降解Chie-qua多糖.
降解后的Chie-qua多糖体表现出改善的生物活性,这表明了潜在的应用.
这项研究支持在食品和医疗行业中使用修饰的Chie-qua多糖.

关键词:

它具有抗铁酶活性.具有抗氧化活性的抗氧化剂.贝尼纳卡萨他的第一个变种. 奇埃克瓦如何如何如何聚糖的降解降解多糖.响应表面方法 (RSM)

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