BmNPV GP64的信号激活了ERAD通路,以调节异质分泌蛋白表达
Na Liu1, Ying Xu1, Luping Sun1
1Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, PR China.
博姆比克斯·莫里核聚合体病毒 (BmNPV) GP64信号通过激活内分泌网膜相关降解 (ERAD) 途径,增强了baculovirus表达系统中的重组蛋白质分泌. 优化ERAD基因可以改善蛋白质分泌和病毒感染性.
科学领域:
- 生物技术是生物技术.
- 分子生物学分子生物学
- 病毒学 病毒学
背景情况:
- 细菌病毒表达载体系统 (BEVS) 对于产生重组蛋白质至关重要.
- 在BEVS中优化膜和分泌蛋白质表达需要进一步的研究.
- 信号在蛋白质分泌和细胞处理中起着关键作用.
研究的目的:
- 调查Bombyx mori核多重体病毒 (BmNPV) GP64信号 (SP) 对重组蛋白表达和分泌的影响.
- 探索内细胞网膜相关降解 (ERAD) 途径在SP介导分泌中的作用.
- 评估ERAD调制对BEVS效率和病毒感染性的影响.
主要方法:
- 在BmN细胞中,具有和没有BmNPV GP64 SP的重组蛋白的短暂表达.
- 蛋白质表达,定位和分泌的定量分析.
- 通过基因表达分析 (BiP,PDI,UFD1,S1P,ASK1) 调查ERAD通路的激活.
- 通过RNA干扰 (RNAi) 来破坏与ERAD相关的基因.
- 用BmNPV进行病毒感染实验.
主要成果:
- BmNPV GP64 SP提高了蛋白质分泌的效率,同时减少了细胞内积累.
- 由SP介导的分泌与ERAD相关基因的表达增加相关,包括BiP.
- 击败ERAD基因,特别是BiP,显著改善了SP引导的蛋白质分泌.
- 在BmNPV感染期间观察到ERAD基因上调,这表明它在病毒处理和传染性中起作用.
结论:
- 在BEVS.中,BmNPV GP64 SP调节蛋白质分泌和ERAD通路激活.
- 调节ERAD通路为优化昆虫细胞中重组蛋白质生产提供了一种策略.
- 了解SP,ERAD和病毒感染力之间的相互作用可以促进BEVS的生物技术应用.
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