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通过直接输注多维质谱法对寡糖类异构体进行区分
Enoch Amoah1, Taghi Sahraeian1, Ayesha Seth1
1Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA. badu-tawiah.1@osu.edu.
The Analyst
|October 18, 2024
概括
现在可以使用质谱学与化物添加物进行分化寡糖化物异构体. 这种先进的技术有助于分析复杂的混合物和潜在的疾病生物标志物.
科学领域:
- 分析化学 分析化学
- 生物化学 生化学
- 质谱测量质量谱测量
背景情况:
- 寡糖类具有多样化的生物活性,并作为疾病生物标志物,使其结构性特征至关重要.
- 寡糖的结构复杂性为准确的表征和同位素分化带来了重大挑战.
- 需要复杂的分析仪器来区分各种寡糖类异构体.
研究的目的:
- 报告化物添加物 (Cl-, Br-) 的使用,用于通过碰撞诱导解离联质谱法 (CID MS/MS) 分化寡糖类异构体.
- 为了证明传统的纳米电子喷雾电离 (nESI) CID MS/MS平台的异构体分析能力.
- 展示该方法在用于诊断应用的复杂混合物和生物流体分析方面的潜力.
主要方法:
- 使用直接输注纳米电子喷雾电离 (nESI) 产生化物添加物 (Cl-, Br-).
- 使用常规碰撞诱导解离 (CID) 串联质谱法 (MS/MS) 分析寡糖类异构体.
- 主要组件分析 (PCA) 的MS/MS碎片离子形状,比较负离子化物添加与正离子添加.
- 复杂的混合物分析,包括将寡糖类入原始尿液,并采用二维分离 (分子量和CID MS/MS).
主要成果:
- 成功分化三糖,四糖和五糖的立体异构体,使用化物添加物和CID MS/MS.
- 通过不同的诊断断片离子,在14种不同的寡糖体中区分位置,结构和链接异构体.
- 负离子模式的化物添加证明在异构体分化方面比正离子模式的添加更有效,正如PCA所示.
- 通过使用二维MS/MS方法,成功地在复杂的原尿基质中区分了多个寡糖类物种.
结论:
- 化物添加物允许使用传统的nESI CID MS/MS直接分化多种类型的寡糖类异构体.
- 这种方法为在反应混合物中表征合成寡糖异构体提供了强大的工具.
- 该技术显示出用于用于诊断目的的生物流体中寡糖的结构分析的巨大潜力.
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