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Updated: May 8, 2026

Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue
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Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue

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一种经过修改的双重准备方法,用于从激光微切割的新鲜冷的人类癌症组织样本中更好地分离核酸

Danielle C Kimble1,2, Tracy J Litzi1,3, Gabrielle Snyder1,3

  • 1Gynecologic Cancer Center of Excellence, Department of Gynecologic Surgery and Obstetrics, Uniformed Services University of the Health Sciences, Walter Reed National Military Medical Center, Bethesda, MD 20889, United States.

Biology methods & protocols
|October 18, 2024
PubMed
概括

这项研究修改了一种双重准备方案,以改善从激光微切割 (LMD) 采集的癌细胞中获得的RNA产量. 改进的方法确保了足够的核酸投入用于分子研究,有助于癌症微环境研究.

关键词:
激光微切割是微切割的方法之一.下一代测序:核酸隔离的核酸测序核酸双重制备的核酸制备

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Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion09:09

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

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DNA Isolation01:24

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...