Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

5
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
5
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.1K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

Nature methods·2026
Same author

Inverse-scattering of absorptive samples via beam propagation.

bioRxiv : the preprint server for biology·2026
Same author

Prevalence and Risk Factors of Deep Spinal Infection after Single-shot Epidural Injections: A Nationwide Cohort Study of 3.8 Million Pain Outpatients.

Anesthesiology·2026
Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

bioRxiv : the preprint server for biology·2026
Same author

Ultrasound-Guided Atelocollagen Injection for Chronic Pain After Spinal Surgery: A Retrospective Cohort Study.

Journal of pain research·2026
Same author

Space-time inverse-scattering of translation-based motion.

Optica·2025
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
查看所有相关文章

相关实验视频

Updated: Jun 10, 2025

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points
09:30

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points

Published on: March 2, 2011

15.7K

作为可切换模块的DMD和微镜头阵列用于光学衍射断层扫描中的照明角度扫描.

Siqi Yang1, Jeongsoo Kim1, Mary E Swartz2

  • 1Department of Electrical and Computer Engineering, University of Texas at Austin, 2501 Speedway, Austin, TX 78712, USA.

Biomedical optics express
|October 18, 2024
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种新的光衍射断层扫描 (ODT) 系统,用于无标签的3D成像. 新系统使用数字微镜装置 (DMD) 和微镜阵列 (MLA) 进行高效,独立的角度扫描,提高图像质量和速度.

更多相关视频

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

11.5K
Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.7K

相关实验视频

Last Updated: Jun 10, 2025

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points
09:30

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points

Published on: March 2, 2011

15.7K
Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

11.5K
Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.7K

科学领域:

  • 生物医学光学 生物医学光学
  • 定量阶段成像技术 定量阶段成像技术
  • 3D显微镜的使用方法

背景情况:

  • 光学衍射断层扫描 (ODT) 提供无标签的生物样品的3D折射率 (RI) 映射.
  • 使用扫描镜的传统ODT系统在速度和机械稳定性方面面临限制.
  • 现有的使用DMD或SLM的可切换ODT系统存在功率低效或虚假衍射顺序的问题.

研究的目的:

  • 开发一种具有增强角度扫描能力的新型非干扰度ODT系统.
  • 为了克服扫描镜和当前可切换ODT系统的局限性.
  • 为了实现高效,高速,无标签的生物标本3D RI成像.

主要方法:

  • 开发一种新型ODT系统,包括数字微镜装置 (DMD) 和微镜阵列 (MLA) 进行角度扫描.
  • 在没有物理扫描组件的情况下,独立控制照明角度.
  • 基于照明角度密度要求优化功率效率.

主要成果:

  • 新的DMD-MLA模块独立生成照明角度,消除了虚假的衍射顺序.
  • 与其他可切换ODT方法相比,该系统展示了优化的功率效率.
  • 使用校准微球验证了定量成像能力.
  • 一个多重散射样本 (斑马鱼胚胎) 的成像取得了成功.

结论:

  • 开发的非干扰度ODT系统为无标签3D成像提供了显著的进步.
  • 新型DMD-MLA模块提供独立的角度控制和提高功率效率.
  • 这项技术对生物样本的高分辨率,高速形态成像具有前景.