相关实验视频

Updated: Jun 10, 2025

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points

Published on: March 2, 2011

作为可切换模块的DMD和微镜头阵列用于光学衍射断层扫描中的照明角度扫描

Siqi Yang¹, Jeongsoo Kim¹, Mary E Swartz²

¹Department of Electrical and Computer Engineering, University of Texas at Austin, 2501 Speedway, Austin, TX 78712, USA.

Biomedical optics express

|October 18, 2024

概括

这项研究引入了一种新的光衍射断层扫描 (ODT) 系统,用于无标签的3D成像. 新系统使用数字微镜装置 (DMD) 和微镜阵列 (MLA) 进行高效,独立的角度扫描,提高图像质量和速度.

科学领域:

生物医学光学生物医学光学
定量阶段成像技术定量阶段成像技术
3D显微镜的使用方法

背景情况:

光学衍射断层扫描 (ODT) 提供无标签的生物样品的3D折射率 (RI) 映射.
使用扫描镜的传统ODT系统在速度和机械稳定性方面面临限制.
现有的使用DMD或SLM的可切换ODT系统存在功率低效或虚假衍射顺序的问题.

研究的目的:

开发一种具有增强角度扫描能力的新型非干扰度ODT系统.
为了克服扫描镜和当前可切换ODT系统的局限性.
为了实现高效,高速,无标签的生物标本3D RI成像.

主要方法:

开发一种新型ODT系统,包括数字微镜装置 (DMD) 和微镜阵列 (MLA) 进行角度扫描.
在没有物理扫描组件的情况下,独立控制照明角度.
基于照明角度密度要求优化功率效率.

主要成果:

新的DMD-MLA模块独立生成照明角度,消除了虚假的衍射顺序.
与其他可切换ODT方法相比,该系统展示了优化的功率效率.
使用校准微球验证了定量成像能力.
一个多重散射样本 (斑马鱼胚胎) 的成像取得了成功.

结论:

开发的非干扰度ODT系统为无标签3D成像提供了显著的进步.
新型DMD-MLA模块提供独立的角度控制和提高功率效率.
这项技术对生物样本的高分辨率,高速形态成像具有前景.

更多相关视频

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

相关概念视频

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...