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柏柏林通过调节PPAR信号通路来改善HepG2细胞中高脂肪诱导的胰岛素抵抗

Lingxiao Zhang¹, Chenghao Yang², Xinyue Ding³

¹Department of Cardiology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Current computer-aided drug design

|October 18, 2024

概括

柏柏林通过增强肝细胞的葡萄糖吸收来改善胰岛素抵抗. 这种效应通过过氧酶增殖器激活受体 (PPAR) 信号通路进行介导,突出了柏柏林的关键机制.

科学领域:

药理学药理学是指药理学的学科.
代谢疾病代谢疾病
分子生物学分子生物学

背景情况:

柏柏林 (BBR),源自Coptis chinensis,以其在糖脂代谢和胰岛素抵抗 (IR) 中的作用而闻名.
精确的分子标和机制是BBR对高脂血症诱导的IR的作用的基础,需要进一步阐明.

研究的目的:

调查Berberine (BBR) 改善高脂肪诱导的胰岛素抵抗的分子标和途径.
验证过氧酶增殖器激活受体 (PPAR) 信号通路在BBR治疗效果中的作用.

主要方法:

使用Pharmmapper,GeneCards和OMIM数据库识别了BBR和糖尿病相关的目标.
构建了蛋白质-蛋白质相互作用网络,并进行了基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径丰富分析.
使用在体外抗胰岛素的HepG2细胞模型,用BBR治疗,以评估葡萄糖消耗和吸收,然后进行分子对接和qPCR分析.

主要成果:

确定了BBR和糖尿病之间的262个重叠目标,在KEGG分析中突出显示了PPAR信号通路.
实验室研究表明,BBR显著增加了胰岛素抵抗HepG2细胞的葡萄糖消耗和吸收.
抑制PPAR通路减弱了BBR对胰岛素抵抗HepG2细胞的有益作用.

结论:

过氧酶增殖器激活受体 (PPAR) 信号通路是贝贝林 (BBR) 作用的关键调解者,可以改善HepG2细胞中高脂肪诱导的胰岛素抵抗.

关键词:

网络药理学网络药理学柏柏林氨酸是什么柏柏林氨酸是什么高脂肪的高脂肪含量.胰岛素耐药性是一种胰岛素耐药性.过氧体增殖器激活的受体.

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