孤儿核受体NR4A1调节人间介质干细胞中的骨质母细胞生成和脂肪生成
Yilan Jin1, Youngho Son2, Insun Song1
1Department of Endocrinology and Metabolism, Ajou University School of Medicine, Suwon, Gyeonggi‑do 16499, Republic of Korea.
Molecular medicine reports
|October 18, 2024
概括
核受体子家族4组A成员1 (NR4A1) 基因抑制骨形成并促进人类骨髓干细胞中的脂肪发育. NR4A1通过调节Notch信号来影响骨质疏松症和脂肪生成.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 干细胞研究 干细胞研究
背景情况:
- 核受体子家族4组A成员1 (NR4A1) 基因与骨质疏松症和脂肪生成有关.
- 了解NR4A1在介酶干细胞分化中的作用对于骨和脂肪组织调节至关重要.
研究的目的:
- 研究NR4A1影响人类骨髓衍生中介细胞干细胞 (BMD-MSCs) 骨质母细胞生成和脂肪生成的机制.
主要方法:
- NR4A1在骨质细胞 (MC3T3-E1) 和脂肪细胞 (3T3-L1) 细胞系中过度表达或被击败,以及在人类BMD-MSC细胞系中 (PCS-500-012).
- 使用性酸酶活性,阿利沙林红色S染色和油红色O染色来评估骨质原和脂肪原的分化.
- 使用RNA测序 (RNA-seq) 和发明途径分析 (IPA) 来识别差异表达的基因和信号通路.
主要成果:
- 抑制NR4A1会增加骨质母细胞发生和化,而过度表达NR4A1会降低它们.
- 抑制NR4A1降低了脂肪生成,而NR4A1过度表达增加了脂肪生成.
- RNA-seq和IPA揭示了诺奇信号调解NR4A1对骨质母细胞形成和脂肪形成的影响,在NR4A1淘汰后减少了类似于大脑的转录辅助激素3的表达.
结论:
- NR4A1抑制骨质母细胞发生并促进人类BMD-MSCs中的脂肪发生.
- NR4A1调节了诺奇信号级联,这表明它在骨质疏松症和脂肪生成的进展中的作用.
更多相关视频
08:34Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
Published on: June 3, 2016
15.0K
10:31Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders
Published on: February 9, 2022
3.2K
相关概念视频
Transducer Mechanism: Nuclear Receptors
1.3K
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
1.3K
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Methods of Nuclear Reprogramming
1.8K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.8K
Osteoclasts in Bone Remodeling
2.8K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
2.8K
Stem Cell Niche
5.0K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
5.0K
TGF - β Signaling Pathway
7.2K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.2K
