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相关概念视频

ATP Driven Pumps I: An Overview01:27

ATP Driven Pumps I: An Overview

8.0K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
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ATP Driven Pumps III: V-type Pumps01:30

ATP Driven Pumps III: V-type Pumps

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V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
3.6K
ATP Driven Pumps II: P-type Pumps01:34

ATP Driven Pumps II: P-type Pumps

4.6K
The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V.
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
4.6K
Primary Active Transport01:29

Primary Active Transport

9.8K
In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction they would...
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High Speed Droplet-based Delivery System for Passive Pumping in Microfluidic Devices
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通过双酶微型进行基质控制的双向.

Bogdan Adrian Nicola1, Mihail N Popescu2, Szilveszter Gáspár1

  • 1International Centre of Biodynamics, 1B Intrarea Portocalelor, 060101 Bucharest, Romania.

ACS applied materials & interfaces
|October 18, 2024
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种新的双酶微,能够实现双向流动. 这项创新使用酶来创建微流体设备的自主,非机械.

关键词:
双向抽是一种双向抽.生物化学控制的流量化学活性表面的化学活性.酶性微型的微型葡萄糖酶微型

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科学领域:

  • 生物医学工程 生物医学工程
  • 微流体学 微流体学
  • 酶学 是一种酶学.

背景情况:

  • 自主小型对于便携式微流体学至关重要.
  • 用酶打印的表面可以产生水力动力流,从而实现非机械送.
  • 现有的酶微缺乏双向流量能力.

研究的目的:

  • 在酶微中引入β-葡萄糖酶以产生强大的向内流量.
  • 开发一种具有双向流量控制的双酶微.
  • 推进多功能,生物相容的微型的开发.

主要方法:

  • 纳入β-葡萄糖酶酶用于细胞质糖诱导的流动.
  • 将β-葡萄糖酶和尿酶集成到一个单一的微补丁中.
  • 根据特定基板的反应生成水力动力流的特征.

主要成果:

  • β-葡萄糖酶促进了强大的向内流动 (2.51 ± 0.56 μm s-1 在 80 mM 细胞质).
  • 双酶微型显示了基质依赖的双向流动.
  • 观察到纤维素 (0.95 ± 0.37 μm s−1 在 20 mM) 的内流,尿素 (1.46 ± 0.47 μm s−1 在 20 mM) 的外流.

结论:

  • β-葡萄糖酶是有效的创建酶微与内流.
  • 新型双酶微型实现了控制的双向流量.
  • 这项技术是按需,生物相容的微流体送的重大进步.