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Updated: May 11, 2026

ReAsH/FlAsH Labeling and Image Analysis of Tetracysteine Sensor Proteins in Cells
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在活细胞中可视化VDAC1使用四氨酸标签.

Johannes Pilic1, Furkan E Oflaz1, Benjamin Gottschalk1

  • 1Gottfried Schatz Research Center, Molecular Biology and Biochemistry, Medical University of Graz, Graz, Austria.

PloS one
|October 18, 2024
PubMed
概括

我们开发了一种使用四半氨酸 (TC) 标记的新方法,用于对电压依赖离子通道1 (VDAC1) 的活细胞成像. 这种技术揭示了线粒体,ER-线粒体接触点和亡期间的VDAC1动态.

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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...