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相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Overview of Electron Microscopy01:25

Overview of Electron Microscopy

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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
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Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

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Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
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相关实验视频

Updated: Jun 10, 2025

Implementation of a Coherent Anti-Stokes Raman Scattering CARS System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
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代谢光吸收,散射和发射 (MetaLASE) 显微镜

Brendon S Restall1, Nathaniel J M Haven1, Matthew T Martell1

  • 1Department of Electrical and Computer Engineering, University of Alberta, 116 Street & 85 Avenue, Edmonton, Alberta T6G 2R3, Canada.

Science advances
|October 18, 2024
PubMed
概括

MetaLASE显微镜为同时可视化组织新陈代谢和组织结构提供了一种新方法. 这种光学成像技术提供虚拟组织学和代谢读数,有助于疾病研究和诊断.

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Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos
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科学领域:

  • 生物医学光学 生物医学光学
  • 分子成像学分子成像学
  • 组织病理学 组织病理学

背景情况:

  • 目前的光学成像缺乏详细的组织结构.
  • 组织学方法缺乏代谢对比.
  • 需要进行综合代谢和结构组织分析.

研究的目的:

  • 为了引入代谢光吸收,散射和发射 (MetaLASE) 显微镜.
  • 为了实现同时进行虚拟组织学和光学代谢读取.
  • 为了改善细胞和组织代谢状态的可视化.

主要方法:

  • 利用光声学遥感进行血素样核对比.
  • 采用紫外线反射显微镜用于以样细胞质对比.
  • 激发内源性自光 (NAD,P,H,FAD,原) 用于使用光学氧化还原比进行代谢映射.

主要成果:

  • 通过MetaLASE显微镜实现了同时进行虚拟组织学和代谢成像.
  • 视觉化代谢变化,包括侵入性癌瘤.
  • 在良性慢性炎症和腺体中观察到过度代谢.

结论:

  • MetaLASE显微镜整合了组织学和代谢信息.
  • 提供了用于业内利分析的潜力.
  • 提供了新的研究应用,用于将代谢活动与组织类型相关联.