通过冷等离子体诱导的与酸的共价结合来消除卵白素的抗原性
Chang Liu1, Pan-Pan Tang2, Xiu-Bin Liu2
1College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Food chemistry
|October 18, 2024
概括
冷血治疗有效地将酸 (GA) 移植到卵蛋白 (OVA) 上,显著降低了其过敏性. 这种方法显示出改变食物蛋白质以尽量减少过敏反应的前景.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
背景情况:
- 卵胺 (OVA) 是一种常见的过敏原.
- 减少蛋白质的过敏性对于食品安全至关重要.
- 像酸 (GA) 这样的多具有潜在的抗氧化和抗过敏特性.
研究的目的:
- 为了研究冷血 (CP) 处理对GA对OVA的共价接种.
- 为了确定OVA上的GA结合点.
- 评估GA移植后OVA抗原性的降低.
主要方法:
- 冷血 (CP) 治疗用于将酸 (GA) 移植到卵蛋白 (OVA) 上.
- 液体染色学-并联质谱学 (LC-MS/MS) 用于确定GA结合部位.
- 酶相关免疫吸收试验 (ELISA) 用于评估IgE结合能力.
主要成果:
- 60秒的CP治疗导致OVA-GA复合体中的GA含量为22.97 ± 1.27 mg/g.
- 在OVA-GA综合体的四个特定区域内确定了12个GA结合点.
- 观察到IgE结合能力显著降低70.83±0.90%.
结论:
- 治疗CP是一种有效的方法,可以将酸共振地移植到卵上.
- 通过掩盖线性表位和破坏构造表位,GA移植显著降低了OVA抗原性.
- 这种方法为减少基于蛋白质的食品成分的过敏性提供了一个可行的策略.
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