相关实验视频
Updated: Jun 10, 2025

08:26
Monitoring Stub1-Mediated Pexophagy
Published on: May 12, 2023
1.5K
外部线粒体膜E3 Ub结合酶MARCH5 控制 de novo 过氧体生物发生
Nicolas Verhoeven1, Yumiko Oshima1, Etienne Cartier1
1Center for Biomedical Engineering and Technology, University Hospital Basel, University of Basel, Basel, Switzerland; Department of Biochemistry and Molecular Biology, University Hospital Basel, University of Basel, Basel, Switzerland.
Developmental cell
|October 18, 2024
概括
在线粒体中,E3泛基因酶MARCH5对于从线粒体中产生过氧体至关重要. 它的缺失会损害过氧体的形成和功能,突出显示MARCH5
科学领域:
- 细胞生物学 细胞生物学
- 线粒体生物学 线粒体生物学
- 过氧体生物生成
背景情况:
- 过氧体是参与各种代谢过程的重要器官.
- 过氧体的生物发生是一个复杂的过程,涉及多种蛋白质因素.
- 线粒体已经涉及到过氧体的形成,但根本的机制尚未完全理解.
研究的目的:
- 为了研究外线粒体膜 (OMM) 相关的E3泛素化酶MARCH5在过氧体生物发生中的作用.
- 阐明MARCH5影响线粒体衍生前氧体生成的分子机制.
主要方法:
- 使用CRISPR-Cas9基因编辑来创建MARCH5淘汰和双淘汰细胞系 (MARCH5/Pex14,MARCH5/Pex3).
- 使用免疫光显微镜可视化过氧体和线粒体,并跟踪蛋白质定位.
- 分析了蛋白质表达水平和无处不在的状态,使用西方抹黑和免疫沉试验.
主要成果:
- 3月5日,淘汰赛细胞表现出不成熟的过氧体和脂肪酸诱导的过氧体生物发生障碍的积累.
- 在脂肪酸刺激过程中,MARCH5被重新分配到过氧体中,其无处不在的缺陷突变体积聚在过氧体前.
- 氧体生物发生因子Pex14的耗尽导致了MARCH5和Tom20阳性前氧体的积累,而MARCH5/Pex14双重淘汰赛中没有检测到氧体.
- 在MARCH5缺乏的细胞中,线粒体功能保持完整,并且观察到过氧体蛋白的表达减少.
结论:
- MARCH5 是一种关键的调节器,对于线粒体衍生的前氧体的生成至关重要.
- MARCH5在线粒体依赖的过氧体生物发生过程中发挥着中心作用,独立于其作为线粒体质量控制因子的功能.
- 这些发现揭示了线粒体动力学和通过MARCH5.5调解的过氧体形成之间的新联系.
相关概念视频
Porin Insertion in the Outer Mitochondrial Membrane
2.8K
Porins are beta-barrel proteins translocated to the mitochondrial outer membrane through the TOM complex into the intermembrane space. Porin precursors bind TIM chaperones within the intermembrane space and are guided to the Sorting and Assembly Machinery complex or SAM complex on the outer mitochondrial membrane.
Three models describe the assembly of porins by the SAM complex and their insertion into the outer membrane. Model 1 suggests that porins are assembled outside the SAM channel as the...
Three models describe the assembly of porins by the SAM complex and their insertion into the outer membrane. Model 1 suggests that porins are assembled outside the SAM channel as the...
2.8K
Translocation of Proteins into the Mitochondria
3.0K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.0K
Protein Import into the Peroxisomes
3.4K
Cells contain membrane-bound organelles called peroxisomes that oxidize organic molecules by transferring hydrogen atoms to oxygen, producing hydrogen peroxide. Peroxisomes enzymatically convert the released hydrogen peroxide into water and oxygen.
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...
3.4K
Structure of Porins
2.9K
Mitochondria, chloroplasts, and gram-negative bacteria have transmembrane, beta-barrel proteins called porins to mediate the free diffusion of ions and metabolites across the membrane. Mitochondrial porin precursors contain conserved amino acid sequences called beta signals at their C-terminal. Beta signals have a motif of PoXGXXHyXHy (Po-Polar, X-Any amino acid, G-Glycine, Hy-LargeHydrophobic), which are crucial for precursor recognition to initiate precursor assembly. Beta-barrel...
2.9K
Peroxisomes
11.1K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
11.1K
Peroxisomes and Mitochondria
86.6K
Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
86.6K

