从废弃的酒酵母中制备和表征不溶性β-葡萄糖
Huaqiang Cao1, Simiao Wen1, Xiaoliu Deng1
1School of Life Sciences, Henan University, Henan Province, Kaifeng 475004, China; Engineering Research Center for Applied Microbiology of Henan Province, Kaifeng 475004, China.
International journal of biological macromolecules
|October 18, 2024
概括
这项研究通过提取高纯度β-葡萄糖 (β-葡萄糖) 来提升废品酒酵母的价值. 优化的提取产生95.84%纯β-葡萄糖具有抗炎性质,为酒行业提供可持续的解决方案.
科学领域:
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
- 环境科学 环境科学
背景情况:
- 酒厂的废物,特别是消耗的酒酵母,带来了环境和经济挑战.
- 对这种废物流的利用对于可持续的工业实践至关重要.
研究的目的:
- 开发一种高效的方法,从废品酒酵母中提取高纯度的β-葡萄糖 (β-葡萄糖).
- 描述提取的β-葡萄糖,并评估其作为功能性食品成分的潜力.
主要方法:
- 酵母自解,然后进行性处理以去除杂质.
- 使用二甲基硫氧化物 (DMSO) 优化β-葡萄糖 (β-葡萄糖) 提取条件.
- 通过薄层染色学 (TLC),里埃变形红外光谱学 (FTIR) 和核磁共振 (NMR) 进行结构分析.
主要成果:
- 通过80°C的0.7%NaOH在2.6小时内获得最佳的杂质去除.
- 最佳的β-葡萄糖 (β-葡萄糖) 提取:80%的DMSO,30:1的固体-液体比率,80°C30分钟.
- 获得了95.84%的纯度和5.56%的β-葡萄糖 (β-葡萄糖) 产量;确认结构为具有β-糖键的葡萄糖聚合物.
- 在体外表现出显著的抗炎性质.
结论:
- 废弃的酒酵母可以有效地利用,产生高纯度的β-葡萄糖 (β-葡萄糖).
- 提取的β-葡萄糖 (β-葡萄糖) 具有抗炎性质,表明功能性食品应用的潜力.
- 这个过程为酒厂废物管理提供了一个可持续和经济可行的解决方案.
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