通过YY1/iNOS通路调节肺上皮细胞中的铁和真菌杀菌活性
Qiuwen Fei1, Jian Zhang2, Liangqiong Chen2
1Department of Pathogen Biology, Medical College, Nantong University, No. 19 Qixiu Road, Nantong, China.
Life sciences
|October 18, 2024
概括
转录因子YY1在肺细胞中感染Mycobacterium marinum时抑制铁. 调节YY1降低了细菌负载,提供了潜在的新结核病治疗方法.
科学领域:
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
背景情况:
- 结核菌菌菌的感染导致宿主细胞死亡,包括肺上皮细胞中的铁亡.
- 转录因子YY1对于调节铁亡至关重要,但其确切的作用尚不清楚.
研究的目的:
- 为了研究YY1在肺上皮细胞中Mycobacterium marinum感染期间ferroptosis中的作用.
- 阐明YY1影响铁和细胞内细菌生存的机制.
主要方法:
- 肺上皮细胞 (A549,H1299) 被M. marinum感染.
- 流细胞计评估了细胞死亡和反应性氧物种 (ROS).
- 殖民地形成单位 (CFU) 试验测量了细菌负荷;分析了铁亡标记物 (MDA,GSH) 和YY1-iNOS促进物相互作用 (双化酶试验).
主要成果:
- M. marinum感染诱导了肺上皮细胞中的铁亡,在8小时达到峰值,并且随着感染次数 (MOI) 的增加而增加.
- YY1敲击减弱了铁亡,并降低了SLC7A11和GPX4的表达.
- YY1过度表达增强了杀菌分子 (iNOS,MPEG1) 的表达,减少了细菌负载,并与iNOS促进体结合.
结论:
- YY1 抑制了M. marinum诱导的铁亡,并减少了肺上皮细胞中的细胞内细菌的增殖.
- 向YY1通过调节宿主细胞死亡途径,为结核病提供了潜在的治疗策略.
关键词:
在1400W的电源中,有1400W.具有杀菌活性的细菌.铁性化 (ferroptosis) 是一种铁素-1-1是一种铁素.肺上皮细胞是肺上皮细胞.YY1 YY1 这是一个很大的问题.我们的 iNOS iNOS更多相关视频
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