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Updated: Jun 10, 2025

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

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闪光量:一个快速的算法,用于蛋白形量化在顶向下的蛋白质组学

Jihyung Kim1,2, Kyowon Jeong1,2, Philipp T Kaulich3

  • 1Applied Bioinformatics, Department for Computer Science, University of Tübingen, 72076 Tübingen, Germany.

Analytical chemistry
|October 18, 2024
PubMed
概括

使用自上而下的蛋白质组学 (TDP) 精确量化蛋白质形式,使用FLASHQuant.改进了. 这种新方法解决了聚合信号,提高了快速LC-MS运行中的量化准确性和可重复性.

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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