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使用生物传感器和流细胞计的自动化酵母培养控制

Raquel Perruca Foncillas¹, Sara Magnusson¹, Basel Al-Rudainy²

¹Division of Applied Microbiology, Department of Chemistry, Lund University, SE-22100 Lund, Sweden.

Journal of industrial microbiology & biotechnology

|October 18, 2024

概括

本研究介绍了微生物生物处理的自动控制系统. 它使用流细胞计和生物传感器来监测酵母细胞并保持最佳条件,即使在furfural的压力下.

科学领域:

生物技术和生物加工
微生物生理学微生物生理学
合成生物学合成生物学

背景情况:

有效的微生物生物处理需要精确控制细胞性能和强度.
传统的监测方法往往缺乏检测微妙生理变化的灵敏度.
像furfural这样的环境压力因素可能会对微生物培养效率产生负面影响.

研究的目的:

评估一个自动化料批量种植控制系统.
为了利用在线流细胞计与还氧化生物传感器监测酵母细胞生理学.
评估系统在毛皮压力下保持细胞健康的能力.

主要方法:

开发一个采用线路流细胞计的食批量种植控制系统.
使用完整的酵母细胞与基于光转录因子的氧化还原生物传感器 (TRX2p-yEGFP).
监测NADPH/NADP+比率,以应对暴露在furfural中的情况.

主要成果:

控制系统成功检测到生物传感器输出,并自动调整毛皮料料速率.
酵母菌株的生理能力即使在高度的furfural中也保持不变.
单细胞测量允许监测子群动态,提高控制精度.

结论:

结合生物传感器和流细胞计的自动控制系统提供了强大的微生物培养.
利用细胞内特性作为控制输入可以提高系统性能.
这种方法具有优化工业生物处理应用的巨大潜力.

关键词:

自动实时流动细胞计量自动化.控制战略控制战略控制战略发酵发酵发酵发酵不同质性的异质性合成生物学合成生物学

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