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Updated: Jun 10, 2025

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
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Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

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从单个人类B细胞克隆抗体的表达

Tim Rollenske1, Rajagopal Murugan2, Hedda Wardemann3

  • 1Institute of Molecular Medicine and Experimental Immunology, University Hospital Bonn, Rheinische Friedrich Wilhelm University, Bonn, Germany.

Methods in molecular biology (Clifton, N.J.)
|October 18, 2024
PubMed
概括

这项研究提出了一种分析人类B细胞抗体活性的新方法. 这项技术有助于了解B细胞受体在淋巴瘤发育和克隆进化中的作用.

关键词:
免疫球蛋白是一种免疫球蛋白.一个单细胞RT-PCR.抗体是对抗体的一种.

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相关概念视频

Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
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