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相关概念视频

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...

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对正常和恶性B细胞单细胞转录组分析.

Antony B Holmes1, Clarissa Corinaldesi1, Katia Basso2

  • 1Institute for Cancer Genetics, Columbia University, New York, NY, USA.

Methods in molecular biology (Clifton, N.J.)
|October 18, 2024
PubMed
概括

单细胞转录组学分析单个细胞中的基因表达,揭示细胞类型和状态. 这项研究将基因表达与B细胞的免疫谱的分析结合在一起,有助于克隆型进化和瘤克隆检测.

科学领域:

  • 免疫学 免疫学 免疫学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 大量分析平均基因表达,掩盖细胞异质性.
  • 单细胞 (sc) 转录组学为单个细胞类型和状态提供了高分辨率的洞察力.
  • 存在多种不同的sc转录技术,每个都有独特的协议.

研究的目的:

  • 分析正常和恶性成熟B细胞使用10×基因组学5'sc基因表达.
  • 整合基因表达数据与B细胞免疫谱的分析.
  • 提供一个全面的指南,用于实验协议和数据分析使用流行的 sc 软件工具.

主要方法:

  • 10× 基因组学 5' sc-基因表达概况.
  • B细胞免疫谱的概况.
  • 生物信息分析整合了基因表达和目录数据.

主要成果:

  • 为不同的细胞群体重建完整的转录组.
  • 鉴定表达的免疫球蛋白基因用于克隆型分析.
  • 检测共享相同B细胞受体序列的瘤克隆.

结论:

关键词:
B细胞受体受体B细胞受体受体发芽中心是生殖中心.淋巴瘤是一种淋巴瘤.在RNA-seqqq.单细胞转录组学 单细胞转录组学

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  • 集成的sc转录组学和免疫谱的分析提升了B细胞分析.
  • 这种方法促进了克隆型进化的研究和恶性细胞检测.
  • 本章为使用sc分析工具的研究人员提供了实际指导.