来自谷氨胺,乳酸盐和糖醇的糖生成和糖核生成支持人类巨细胞的功能
Najia Jeroundi1, Charlotte Roy1, Laetitia Basset1
1Univ Angers, Nantes Université, Inserm, CNRS, CRCI2NA, SFR ICAT, LabEx IGO, F-49000, Angers, France.
EMBO reports
|October 18, 2024
概括
人类巨细胞利用糖原来获得能量,特别是依赖于糖核生成的炎症性M1细胞. 这种储存的能量为抗击感染和组织修复等基本功能提供燃料.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞的新陈代谢
背景情况:
- 巨细胞对于宿主防御和组织修复至关重要,通常在营养有限的环境中起作用.
- 了解巨细胞代谢途径是理解它们多样化的作用的关键.
研究的目的:
- 研究人类巨细胞代谢糖原的能力.
- 为了确定涉及巨细胞糖核生成的基质和途径.
主要方法:
- 通过细胞因子 (GM-CSF,M-CSF,IL-4,IFNγ) 刺激单细胞衍生的巨细胞.
- 用酶抑制,基因沉默和13C追踪来评估糖生成和糖核生成.
- 分析了包括TCA循环和酸途径 (PPP) 在内的代谢途径.
主要成果:
- 细胞因子诱导了葡萄糖生成和葡萄糖在巨细胞中的积累.
- 炎症性M1巨细胞通过PCK2和FBP1.1利用糖原生成.
- 谷氨胺,乳酸和甘油作为M1细胞中的糖原生成基质.
- 与瘤相关的巨细胞 (TAMs) 也储存和代谢糖原.
- 巨细胞糖原分解和PPP支持功能独立于外部葡萄糖.
结论:
- 巨细胞的糖原代谢支持M1和M2细胞的功能.
- 炎症性M1巨体显示出一种潜在的依赖于糖原生成的能量.
- 糖原是巨细胞效应因子功能的关键能量来源.
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