动物中菌微生物群和抗生素耐药性的元基因组概况
Kai-Meng Shang1, Hany M Elsheikha2, He Ma1
1College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province, PR China.
Ecotoxicology and environmental safety
|October 19, 2024
概括
动物肠道细菌含有许多抗生素耐药性基因 (ARG),特别是针对多种药物,糖和埃尔法米辛的耐药性. 大多数ARG都被发现在奥斯基洛斯皮拉家族中,突出突出了动物菌群作为一个关键的储存库.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 环境科学 环境科学
背景情况:
- 动物肠道微生物群是抗菌素耐药性 (AMR) 的重要储存库.
- 在动物体群体中,抗生素耐药性基因 (ARG) 的分布和宿主尚未得到充分理解.
- 了解野生动物中的ARG患病率对于监测AMR传播至关重要.
研究的目的:
- 为了全面描述五种动物物种的脑内微生物群中的ARG多样性和分布.
- 为了识别携带这些ARG的特定细菌物种.
- 研究微生物群落结构与抗体之间的关系.
主要方法:
- 122 个动物头部样本的高通量测序.
- 构建一种动物菌微生物组基因目录.
- 基于元基因组组的宿主跟踪和与移动遗传元素的相关性分析.
主要成果:
- 在32个耐药性类别中确定了3703个假定ARG蛋白编码基因 (392种独特类型).
- 主导类是Bacillota和Bacteroidota,物种之间存在差异.
- 大多数ARG与多种药物,糖和埃尔法米辛耐药性有关.
- 被确定为ARG的主要宿主家族. Oscillospiraceae.
- 130个ARG与移动遗传元素有显著的相关性.
结论:
- 动物状微生物群含有多种ARG,其中多药耐药性基因最为普遍.
- 螺杆菌 (Oscillospiraceae) 细菌是动物肠道内ARG的主要载体.
- 微生物社区结构和抗体结构之间的相关性是显著的.
- 这些发现强调了动物相关微生物群在抗菌素耐药性生态中的重要性.
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