铜 (II) 复合物含有水和双二/类联体,用于抗癌应用,对抗乳腺癌细胞
Dorothy Priyanka Dorairaj1, Prashant Kumar1, Haritha Rajasekaran1
1Department of Chemistry, National Institute of Technology, Tiruchirappalli 620015, India.
Journal of inorganic biochemistry
|October 19, 2024
概括
新的铜 (II) 复合物与水和类联体显示出强大的抗癌活性,对抗乳腺癌细胞. 最活跃的复合物诱导T47D细胞的亡和G0/G1细胞周期停止.
科学领域:
- 协调化学 协调化学
- 药用化学 医学化学
- 生物物理化学 生物物理化学
背景情况:
- 混合联体铜 (II) 复合物被探索其生物应用.
- 水和双二/类联体提供了多样化的协调可能性.
研究的目的:
- 合成和表征新的混合联结体Cu (II) 复合体.
- 研究它们与DNA和BSA的相互作用.
- 评估它们对乳腺癌细胞系的体外抗癌活性.
主要方法:
- 光谱和分析特征 (例如,X射线晶体学).
- 使用吸收和发射光谱学的DNA/BSA相互作用研究.
- 针对MCF7,T47D,MDA MB 231和MCF 10a细胞系进行体外细胞毒性测试 (IC50测定).
- 亡试验 (AO/EB,DAPI,Annexin-V FITC染色) 进行.
- 细胞周期分析.
主要成果:
- 合成和表征了五种双氨酸 (CB1-CB5) 和五种氨酸 (CP1-CP5) 复合体.
- CB1的X射线结构证实了Cu (II) 周围扭曲的正方形金字塔几何.
- 与双胺复合物相比,类复合物 (CP1-CP5) 显示出更高的抗癌活性.
- 复杂的CP1显示出对T47D细胞的显著活性 (IC50 = 5.8 ± 0.3μM).
- 在T47D细胞中,CP1诱导的亡和G0/G1细胞周期停止.
结论:
- 混合带Cu (II) 复合物,特别是那些含有类素的复合物,是有前途的抗癌药物.
- 复杂的CP1通过诱导亡来表现出对T47D乳腺癌细胞具有强大和特异性的活性.
- 进一步调查CP1作为潜在的治疗剂是有必要的.
![[DPEPhosbcpCu]PF6: A General and Broadly Applicable Copper-Based Photoredox Catalyst](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F59739.jpg&w=3840&q=50)
相关概念视频
02:57Coordination Number and Geometry
15.6K
For transition metal complexes, the coordination number determines the geometry around the central metal ion. Table 1 compares coordination numbers to molecular geometry. The most common structures of the complexes in coordination compounds are octahedral, tetrahedral, and square planar.
15.6K
00:43Complexometric Titration: Ligands
918
Different monodentate and polydentate ligands are used as complexing agents in complexometric titration reactions. The formation of complexes by mono- and bidentate ligands involves two or more intermediate steps, limiting their use as complexing agents. In comparison, polydentate ligands can form complexes with metal ions in a single-step process, facilitating sharper end points. This means polydentate ligands, such as amino carboxylic acid derivatives, are most commonly employed in...
918
00:56Extraction: Advanced Methods
425
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
425
02:42Valence Bond Theory
8.5K
Coordination compounds and complexes exhibit different colors, geometries, and magnetic behavior, depending on the metal atom/ion and ligands from which they are composed. In an attempt to explain the bonding and structure of coordination complexes, Linus Pauling proposed the valence bond theory, or VBT, using the concepts of hybridization and the overlapping of the atomic orbitals. According to VBT, the central metal atom or ion (Lewis acid) hybridizes to provide empty orbitals of suitable...
8.5K
03:02Colors and Magnetism
11.6K
Color in Coordination Complexes
When atoms or molecules absorb light at the proper frequency, their electrons are excited to higher-energy orbitals. For many main group atoms and molecules, the absorbed photons are in the ultraviolet range of the electromagnetic spectrum, which cannot be detected by the human eye. For coordination compounds, the energy difference between the d orbitals often allows photons in the visible range to be absorbed and emitted, which is seen as colors by the human...
When atoms or molecules absorb light at the proper frequency, their electrons are excited to higher-energy orbitals. For many main group atoms and molecules, the absorbed photons are in the ultraviolet range of the electromagnetic spectrum, which cannot be detected by the human eye. For coordination compounds, the energy difference between the d orbitals often allows photons in the visible range to be absorbed and emitted, which is seen as colors by the human...
11.6K
01:19Phase II Reactions: Miscellaneous Conjugation Reactions
47
Phase II biotransformations are detoxification mechanisms that conjugate xenobiotics with endogenous substances, neutralizing their toxicity.
A key example involves the conjugation of cyanide ions, which impair cellular respiration and alter hemoglobin into non-oxygen-carrying cyanmethemoglobin. To neutralize this threat, a sulfur atom from thiosulphate is transferred to the cyanide ion, catalyzed by the enzyme rhodanese, resulting in an inactive compound called thiocyanate. The production of...
A key example involves the conjugation of cyanide ions, which impair cellular respiration and alter hemoglobin into non-oxygen-carrying cyanmethemoglobin. To neutralize this threat, a sulfur atom from thiosulphate is transferred to the cyanide ion, catalyzed by the enzyme rhodanese, resulting in an inactive compound called thiocyanate. The production of...
47