循环中的细胞外囊载荷的变化与大手术后的认知衰退有关:一项观察性病例对照研究
Souren Mkrtchian1, Maria Eldh2, Anette Ebberyd1
1Department of Physiology and Pharmacology, Section for Anaesthesiology and Intensive Care Medicine, Karolinska Institutet, Stockholm, Sweden.
British journal of anaesthesia
|October 19, 2024
概括
手术影响细胞外囊泡 (EVs) 和神经认知. 关节/膝关节置换术后的不良结果与改变的EV微RNA和补充系统蛋白有关,这表明EV是潜在的生物标志物.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 手术后神经认知衰退是一种常见的并发症,其机制不清楚.
- 细胞外囊泡 (EVs) 是外围和大脑之间的潜在信使.
- 这项研究调查了部或膝盖置换手术后的EV变化.
研究的目的:
- 为了检查关节或膝关节置换手术对循环EV组成的影响.
- 为了将EV变化与手术后3个月的神经认知结果相关联.
- 探索EVs,特别是补充系统蛋白质在神经认知衰退中的作用.
主要方法:
- 分析了来自12名患者的血衍生的EVs的microRNAome和proteome (6个好,6个糟糕的神经认知结果).
- 在手术前和手术后的多个时间点 (4,8,24,48小时) 采集样本.
- 通过流式细胞计量证实了补充C3-EV关联,并通过ELISA测量了C3/C3a度.
主要成果:
- 八个失调的EV微RNA (miRNA) 仅在不良神经认知结果组中被发现.
- 观察到免疫相关的EV蛋白增加,包括补充C3,在不良结果组中更早的动力学.
- 脑脊液 (CSF) 衍生的EV在48小时后在不良结果组中显示出C3的显著下降,与CSF的补充激活.
结论:
- 手术显著改变了血和脑脊液衍生的EVs,特别是在神经认知结果不佳的患者中.
- 电脑,特别是那些涉及补体系统的电脑,显示出作为预测神经认知衰退的生物标志物的潜力.
- 需要更大的队列研究来验证这些发现.
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